<p>In recent scenarios, recycling waste and reducing environmental pollution are primary goals of sustainable development. Sustainable development in structural materials is currently gaining global attention. Utilizing solid waste, construction and demolition debris, and natural resources, along with their reuse, are key strategies for promoting sustainability in the construction industry. Human Hair Fiber with a diameter of 80 μm and a length of 30–40 mm is used as a fiber. Many researchers are developing innovative techniques in concrete technology by incorporating waste materials into concrete. This study focuses on evaluating the effect of using human hair, a waste material, as fiber reinforcement in concrete. Human hair was added at 0%, 1%, 2%, 3%, and 4% by volume of cement to assess its influence on the fresh, physical, and mechanical properties of concrete. Concrete specimens, including cubes, cylinders, and prisms, were cast and tested after curing for 7, 28, 56, and 90 days. The results indicated that the compressive strength increased by 8.05% at 1% human hair content after 28 days. Furthermore, the indirect tensile strength and flexural strength improved by 20.89% and 11.98%, respectively, at 2% human hair content after 28 days. The density of concrete decreased as the human hair content increased, while water absorption also increased with higher hair content after 28 days. Drying shrinkage decreased notably over time. However, the slump value decreased with increasing amounts of human hair.</p>