<p>Heart disease is the top cause of death worldwide, with heart attacks causing irreparable damage to cardiac tissue. Scar tissue formation lowers the heart’s pumping function; hence early diagnosis of muscle injury is critical for timely intervention. The purpose of this study is to construct and test a multilayer antenna capable of detecting heart muscle damage by distinguishing between normal and injured cardiac tissue. A multilayer antenna was designed and tested with ex-vivo chicken heart tissues as a tissue model. The antenna’s performance was evaluated using models and experimental measurements, with gain, efficiency, radiation patterns, voltage standing wave ratio (VSWR), specific absorption rate (SAR), and reflection coefficient (S11) being the primary metrics. The simulation results showed antenna resonance near the MICS band at 420&#xa0;MHz, with an S11 value of −18.6 dB for normal cardiac tissue. The experimental results showed reflection coefficients of −9 dB for normal tissue and − 6 dB for damaged tissue. These changes demonstrate the antenna’s sensitivity to tissue conditions. The proposed multilayer antenna exhibits considerable potential as a diagnostic instrument for identifying damage to the heart muscle. It provides encouraging opportunities for non-invasive cardiac health monitoring, possibly facilitating early identification and better patient outcomes, and it operates efficiently in the MICS frequency spectrum.</p>