<p>Whole blood is the most complex component and a vital reflection of the diseases present in the body. Rapid analysis of blood components is essential for disease detection. Optical sensing technology offers the development of a lab-on-a-chip environment, enabling rapid analysis of blood components. We propose a novel method to detect blood components using a dual-source multi-wavelength photonic crystal nano sensor. This novel sensor will enable multiple blood component detection, which will reduce the time required and, cost of laboratory tests. We have studied the light and bio matter interaction with dual light sources propagating in the proposed sensor design. The designed sensor is a unique combination of two waveguides coupled by ring resonators, providing a unique refractive-index profile and bio-matter light interaction. This unique design of the sensor and the refractive index, which is a function of the wavelength, provides controlled mixing of light. An FDTD simulation was carried out, and this mixing of light for multiple wavelengths from optical windows was analyzed. We measured the peaks at different combinations of wavelengths and analyzed the mixing of light in the photonic crystal sensor. The presence of any blood component in whole blood changes its refractive index, changing the way the light mixes through the sensor. This enables the detection of the presence of blood components. The designed sensor detects the presence of blood serum and plasma and hemoglobin concentrations, corresponding to whole blood, measured at different wavelengths. From the flux obtained after the FDTD simulation, we calculated the sensitivity, Figure of merit, signal-to-noise ratio, Limit of Detection, and Q factor, which were found to be 14485.67952&#xa0;nm/RIU, 805.3048583, 49.59 dB, 0.000062, and 76.97909, respectively. The designed sensor can be further developed to detect multiple blood components.</p>