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<p>The urgency for cost-effective, rapid, and user-friendly tools for detecting biomolecules is critical due to their essential role in biosensors relying on giant magnetoresistance (GMR) technology. This article presents the performance of a novel configuration for α-amylase assay as the target biomolecule by employing the ICs-based dual chip commercial GMR sensor, which offers a straightforward approach with immediate electrical data output. Magnetite (Fe<sub>3</sub>O<sub>4</sub>) magnetic nanoparticles were synthesized using a green approach, employing leaf extract from <i>Moringa oleifera</i>. Meanwhile, the PEG-functionalized green-synthesized Fe<sub>3</sub>O<sub>4</sub> magnetic nanoparticles (Fe<sub>3</sub>O<sub>4</sub>/PEG) were used as a magnetic label and produced in an aqua-solution mixture. Each sensor element surface of the dual chip configuration was then exposed to a minute droplet of analyte solution. Each sensor element surface of the dual chip configuration received a little droplet of analyte solution. As a comparison, single chip configuration was also investigated. The microcontroller was connected to the chip, serving as a source-meter unit, facilitating the expeditious production of measurement results. The sensor system demonstrates a consistent and reliable signal that is well-linear to the α-amylase concentration of interest. In a low biasing magnetic field, the signal can be acquired in just 30&#xa0;s. The dual configuration demonstrated a higher sensitivity in detecting α-amylase (0.98&#xa0;mV/%) compared to the single chip configuration (0.94&#xa0;mV/%). A low limit of detection of 3% was also attained. The sensor demonstrated good repeatability and stability, as evidenced by relative signal deviation ranges of 1–20% over 30&#xa0;s. The dual chip configuration in GMR biosensors is a reliable method for achieving competitive performance with green-synthesized magnetic labels using a low-bias magnetic field. This configuration encourages more responsive detection. The results demonstrate that a facile and fast biomolecule assay method can be achieved by combining the use of a dual chip configuration of GMR sensor and Fe<sub>3</sub>O<sub>4</sub>/PEG with a microcontroller.</p>

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Facile and Fast Assay of Biomolecule Using ICs-Based Giant Magnetoresistance Chip Sensor with Green-Synthesized Magnetite Nanotag

  • 期刊论文

  • 发表日期：

    2025年5月27日

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  • Harlina Ardiyanti,
  • Ni’matil Mabarroh,
  • Nur Aji Wibowo,
  • Nurul Imani Istiqomah,
  • Rivaldo Marsel Tumbelaka,
  • Mahardika Yoga Darmawan,
  • Zurnansyah Zurnansyah,
  • Yuliyan Dwi Prabowo,
  • Moh. Adhib Ulil Absor,
  • Edi Suharyadi

摘要

The urgency for cost-effective, rapid, and user-friendly tools for detecting biomolecules is critical due to their essential role in biosensors relying on giant magnetoresistance (GMR) technology. This article presents the performance of a novel configuration for α-amylase assay as the target biomolecule by employing the ICs-based dual chip commercial GMR sensor, which offers a straightforward approach with immediate electrical data output. Magnetite (Fe3O4) magnetic nanoparticles were synthesized using a green approach, employing leaf extract from Moringa oleifera. Meanwhile, the PEG-functionalized green-synthesized Fe3O4 magnetic nanoparticles (Fe3O4/PEG) were used as a magnetic label and produced in an aqua-solution mixture. Each sensor element surface of the dual chip configuration was then exposed to a minute droplet of analyte solution. Each sensor element surface of the dual chip configuration received a little droplet of analyte solution. As a comparison, single chip configuration was also investigated. The microcontroller was connected to the chip, serving as a source-meter unit, facilitating the expeditious production of measurement results. The sensor system demonstrates a consistent and reliable signal that is well-linear to the α-amylase concentration of interest. In a low biasing magnetic field, the signal can be acquired in just 30 s. The dual configuration demonstrated a higher sensitivity in detecting α-amylase (0.98 mV/%) compared to the single chip configuration (0.94 mV/%). A low limit of detection of 3% was also attained. The sensor demonstrated good repeatability and stability, as evidenced by relative signal deviation ranges of 1–20% over 30 s. The dual chip configuration in GMR biosensors is a reliable method for achieving competitive performance with green-synthesized magnetic labels using a low-bias magnetic field. This configuration encourages more responsive detection. The results demonstrate that a facile and fast biomolecule assay method can be achieved by combining the use of a dual chip configuration of GMR sensor and Fe3O4/PEG with a microcontroller.