An Experimental and Computational Investigation of the Non-covalent Interaction Between Erythrosine B-BSA and Tween Surfactants
摘要
In this work, we report the role of three Tween surfactants in the food dye Erythrosine B (EB) and BSA interaction. The surfactants were Tween 20, Tween 40, and Tween 80. The sensing action of this fluorescein dye was monitored after combining it with BSA and Tween surfactants. The synergistic effect of EB, BSA, and Tween surfactant systems was investigated through measurements of zeta potential, fluorescence, dynamic light scattering (DLS), and UV–visible spectroscopy. From the UV absorbance values, The binding constants of EB + BSA + Tween 20/40/80 systems as calculated using the B–H equation demonstrated 340.13 M−1, the highest for Tween 20 as compared to Tween 40 with 106.38 M−1, and Tween 80 having 21.89 M−1. A similar interaction pattern was observed when the three tween surfactant molecules docked with BSA. The three Tween surfactants enhanced the binding between EB and BSA, and Tween 20 had the maximum impact. The better binding of EB to BSA in the presence of Tween surfactants will lead to less dye concentration in blood plasma which in turn can reduce the adverse effects of the dye in the human body. This work will help to tune EB-protein interactions using surfactants to be used as sensors. To the best of our knowledge, this is the first report to identify the role of tween surfactants in EB-BSA interaction.