<p>This article introduces the concept of pervasive ambiguity about gender to illuminate how individuals experience the destabilization of gender norms in contemporary society. Specifically, it considers how the increasing visibility of transgender and nonbinary identities, alongside broader transformations in the social organization of gender, have unsettled once-stable gender schemas. For many people, this change has produced a sense of pervasive ambiguity—an affective and cognitive experience of disorientation in which individuals struggle to interpret gendered meanings, locate themselves in gendered contexts, and resolve fundamental questions about identity and social norms. Building on Durkheim’s theory of anomie and Ball-Rokeach’s concept of pervasive social ambiguity, this article theorizes <i>pervasive ambiguity about gender</i> as a transitional, situational experience of sociocultural flux. It then operationalizes the concept through the development and validation of the Pervasive Ambiguity About Gender (PAAG) scale in a pair of studies conducted among adults in the U.S. The first study (<i>N</i> = 491) uses exploratory factor analysis to derive a reliable, nine-item scale. The second study (<i>N</i> = 501) then validates the PAAG scale’s factor structure using confirmatory factor analysis and demonstrates the scale’s convergent, discriminant, concurrent, and predictive validity. Together, these studies offer a novel conceptual framework and robust empirical tool for analyzing how individuals register and respond to sociocultural transformations in gender.</p>