<p>Research on entrepreneurial ecosystems for sustainability (EEfS) is rapidly growing. However, research lacks integrative efforts and is marked by considerable theoretical diversity, potentially impeding further development. This study synthesized EEfS research to advance the theory and establish a research agenda, by conducting a theory-driven systematic literature review which builds on agencement theory. This study makes two important contributions. First, it integrates the literature and identifies three crucial themes: value, transformation, and boundaries. Second, it uses agencement theory to rethink the identified themes. Thus, it complements and extends current EEfS research and provides novel theoretical contributions. Moreover, this study provides a conceptual model of EEfS, derives a research agenda, and offers practical implications.</p>