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<p>In this research, Pseudomonas aeruginosa bacteria were used as a&#xa0;reducing agent in the green approach of producing copper oxide nanoparticles. The characteristics of the prepared particles were confirmed using various techniques, including UV-Vis analysis, where the highest absorption peak was found at 288 nm and a&#xa0;1.9 eV energy gap was determined. X‑ray diffraction analysis calculated the size of crystals using the Scherer equation, showing an average crystalline size of 28.5867 nm. Atomic force microscopy revealed a&#xa0;grain size of 43.42 nm. The sample quality was verified using energy dispersive X‑ray spectroscopy, which revealed a&#xa0;high-purity substance devoid of contaminants. Using Fourier transform infrared spectroscopy, the presence of a&#xa0;Cu–O bond was established at 603 cm<sup>–1</sup>.</p>

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Synthesis and characterization of copper oxide nanoparticles utilizing Pseudomonas aeruginosa bacteria

  • 期刊论文

  • 发表日期：

    2026年6月2日

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  • Ali Sh. Maktoof,
  • Laith A. Yaaqoob,
  • Ghuson H. Mohammed

摘要

In this research, Pseudomonas aeruginosa bacteria were used as a reducing agent in the green approach of producing copper oxide nanoparticles. The characteristics of the prepared particles were confirmed using various techniques, including UV-Vis analysis, where the highest absorption peak was found at 288 nm and a 1.9 eV energy gap was determined. X‑ray diffraction analysis calculated the size of crystals using the Scherer equation, showing an average crystalline size of 28.5867 nm. Atomic force microscopy revealed a grain size of 43.42 nm. The sample quality was verified using energy dispersive X‑ray spectroscopy, which revealed a high-purity substance devoid of contaminants. Using Fourier transform infrared spectroscopy, the presence of a Cu–O bond was established at 603 cm–1.