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<p>This review considers the results of research carried out in the past years at the Division of Chemical Enzymology of the Department of Chemistry of the Lomonosov Moscow State University on the development of bioluminescent test systems and their applications in ecology and biotechnology. Luminous bacteria were used as bioindicators in the screening of a large group of mycotoxins and for the selection of enzymes for their detoxification. Cells expressing pH-resistant thermostable firefly luciferase from <i>Luciola mingrelica</i> were used for the first time to study the kinetics and mechanisms of action of various antibiotics and other lytic agents on viable cells of prokaryotic and eukaryotic cells.</p>

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Bioluminescent test systems based on viable cells expressing bacterial luciferase or firefly luciferase

  • 期刊论文

  • 发表日期：

    2025年8月1日

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  • N. N. Ugarova,
  • G. Yu. Lomakina

摘要

This review considers the results of research carried out in the past years at the Division of Chemical Enzymology of the Department of Chemistry of the Lomonosov Moscow State University on the development of bioluminescent test systems and their applications in ecology and biotechnology. Luminous bacteria were used as bioindicators in the screening of a large group of mycotoxins and for the selection of enzymes for their detoxification. Cells expressing pH-resistant thermostable firefly luciferase from Luciola mingrelica were used for the first time to study the kinetics and mechanisms of action of various antibiotics and other lytic agents on viable cells of prokaryotic and eukaryotic cells.