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<p>A microwave-assisted method was developed for rapid synthesis of histidine-functionalized copper nanoclusters (His@CuNCs) as fluorescent probes for vitamin B12 (VB12) detection. This method uses histidine as a stabilizer and ascorbic acid as a reducing agent, enabling single-step green synthesis within 1&#xa0;min, much faster than traditional methods. The nanomaterial exhibited intense blue–green fluorescence (λem = 466&#xa0;nm) with quantum yield of 10.38%. Moreover, the His@CuNCs showed exceptional environmental stability, maintaining their optical properties under varying ionic strengths, pH conditions, and prolonged UV exposure. Upon VB12 introduction, specific electron transfer from VB12 to His@CuNCs caused fluorescence quenching via static quenching and IFE mechanisms. The probe demonstrated a linear response from 0.5 to 270&#xa0;μM with 0.033&#xa0;μM LOD, outperforming most of existing nanocluster-based sensors. The platform demonstrated practical utility of determination of vitamin B12 in vitamin drink and bovine serum samples, and sensing temperature.</p>

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One-step ultrafast preparation of copper nanoclusters with histidine ligand for fluorescent detection of vitamin B12 in food and biological samples

  • 期刊论文

  • 发表日期：

    2025年11月18日

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  • Zhizhou Pan,
  • Maolin Ran,
  • Hui Wu,
  • Yi Zhang

摘要

A microwave-assisted method was developed for rapid synthesis of histidine-functionalized copper nanoclusters (His@CuNCs) as fluorescent probes for vitamin B12 (VB12) detection. This method uses histidine as a stabilizer and ascorbic acid as a reducing agent, enabling single-step green synthesis within 1 min, much faster than traditional methods. The nanomaterial exhibited intense blue–green fluorescence (λem = 466 nm) with quantum yield of 10.38%. Moreover, the His@CuNCs showed exceptional environmental stability, maintaining their optical properties under varying ionic strengths, pH conditions, and prolonged UV exposure. Upon VB12 introduction, specific electron transfer from VB12 to His@CuNCs caused fluorescence quenching via static quenching and IFE mechanisms. The probe demonstrated a linear response from 0.5 to 270 μM with 0.033 μM LOD, outperforming most of existing nanocluster-based sensors. The platform demonstrated practical utility of determination of vitamin B12 in vitamin drink and bovine serum samples, and sensing temperature.