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<p>Zinc is an essential micronutrient which must be obtained through dietary intake or supplementation, while its biological accessibility in the human body is limited by phytic acid and others. To develop highly bioavailable zinc supplements, chestnut protein hydrolysate (CPH) was used to prepare a novel chestnut protein hydrolysate-zinc chelate (CPH-Zn), and the zinc chelation efficiency of CPH-Zn was 53.85 ± 5.31%. Characterization results revealed that zinc binding induced the conformational changs in CPH by coordinating with carboxy oxygen, nitrogen atoms of amide, and nitrogen atoms of amino groups. In addition, zinc chelation significantly altered the surface charge of peptide, and the chelate exhibited a porous network with increased surface roughness. Moreover, the CPH-Zn demonstrated superior antioxidant activities and low cytotoxicity at concentrations below 0.6&#xa0;mg/mL. Notably, during simulated gastrointestinal digestion, zinc solubility of CPH-Zn (17.14 ± 1.91%) was significantly higher than that of ZnSO<sub>4</sub> (6.73 ± 0.56%). These results presented the transformation of a chestnut by-product into CPH-Zn as a safe chelate that overcame bioaccessibility limitations, providing an effective zinc supplementation in human nutrition and animal feed.</p>

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A Novel Chestnut Protein Hydrolysate-Zinc Chelate: Chelation Mechanism, Antioxidant, Bioaccessibility and Cytotoxicity

  • 期刊论文

  • 发表日期：

    2026年2月26日

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  • Minhui Luo,
  • Wen Zhang,
  • Sien Zheng,
  • Fang Yang,
  • Chenyang Xie

摘要

Zinc is an essential micronutrient which must be obtained through dietary intake or supplementation, while its biological accessibility in the human body is limited by phytic acid and others. To develop highly bioavailable zinc supplements, chestnut protein hydrolysate (CPH) was used to prepare a novel chestnut protein hydrolysate-zinc chelate (CPH-Zn), and the zinc chelation efficiency of CPH-Zn was 53.85 ± 5.31%. Characterization results revealed that zinc binding induced the conformational changs in CPH by coordinating with carboxy oxygen, nitrogen atoms of amide, and nitrogen atoms of amino groups. In addition, zinc chelation significantly altered the surface charge of peptide, and the chelate exhibited a porous network with increased surface roughness. Moreover, the CPH-Zn demonstrated superior antioxidant activities and low cytotoxicity at concentrations below 0.6 mg/mL. Notably, during simulated gastrointestinal digestion, zinc solubility of CPH-Zn (17.14 ± 1.91%) was significantly higher than that of ZnSO4 (6.73 ± 0.56%). These results presented the transformation of a chestnut by-product into CPH-Zn as a safe chelate that overcame bioaccessibility limitations, providing an effective zinc supplementation in human nutrition and animal feed.