Scholarlink

<p>High-quality protein and bioactive compounds in quinoa (<i>Chenopodium quinoa</i> Willd) have spotlighted its potential as a functional food ingredient due to its antioxidant, anti-inflammatory, and immunomodulatory effects. This study investigates the immunomodulatory potential of quinoa protein concentrate (QPC), quinoa protein hydrolysate (QPH), and a quinoa peptide fraction (QPF &lt; 3&#xa0;kDa) for activating macrophages. QPH was prepared via alcalase hydrolysis of QPC, followed by ultrafiltration (QPF &lt; 3&#xa0;kDa). In vitro and ex vivo assays on mice peritoneal and spleen macrophages were conducted to evaluate the effect of QPC and QPH on cytotoxicity, cytokine profiles (Interleukin (IL)-6, IL-10, Tumour Necrosis Factor (TNF)-α, Interferon (IFN)-γ), and phagocytic activity of macrophages induced by QPC, QPH and QPF. Results indicated that QPC and QPH showed no cytotoxic effects at protein concentrations ≤ 1000&#xa0;µg/mL. QPH at1000 µg/mL increased the production of IFN-γ and TNF-α, while increasing IL-10 release, suggesting a balanced immunostimulant response. Furthermore, QPF significantly enhanced phagocytic activity in spleen macrophages, emphasizing its role in systemic immune activation. These findings suggest quinoa-derived proteins and peptides hold promise as functional ingredients for immune health applications.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Quinoa Protein Hydrolysate as Potential Immunomodulators: Effects on Cytokine Production and Macrophage Activation

  • 期刊论文

  • 发表日期：

    2025年2月22日

查看全文
  • Julio Rueda,
  • Carolina Maldonado Galdeano,
  • Manuel Oscar Lobo,
  • Norma Cristina Sammán

摘要

High-quality protein and bioactive compounds in quinoa (Chenopodium quinoa Willd) have spotlighted its potential as a functional food ingredient due to its antioxidant, anti-inflammatory, and immunomodulatory effects. This study investigates the immunomodulatory potential of quinoa protein concentrate (QPC), quinoa protein hydrolysate (QPH), and a quinoa peptide fraction (QPF < 3 kDa) for activating macrophages. QPH was prepared via alcalase hydrolysis of QPC, followed by ultrafiltration (QPF < 3 kDa). In vitro and ex vivo assays on mice peritoneal and spleen macrophages were conducted to evaluate the effect of QPC and QPH on cytotoxicity, cytokine profiles (Interleukin (IL)-6, IL-10, Tumour Necrosis Factor (TNF)-α, Interferon (IFN)-γ), and phagocytic activity of macrophages induced by QPC, QPH and QPF. Results indicated that QPC and QPH showed no cytotoxic effects at protein concentrations ≤ 1000 µg/mL. QPH at1000 µg/mL increased the production of IFN-γ and TNF-α, while increasing IL-10 release, suggesting a balanced immunostimulant response. Furthermore, QPF significantly enhanced phagocytic activity in spleen macrophages, emphasizing its role in systemic immune activation. These findings suggest quinoa-derived proteins and peptides hold promise as functional ingredients for immune health applications.