Scholarlink

<p>The relationship between the gut microbiota (GM) and the health of human beings has been a topic of growing interest in the last few years. Legumes are a rich source of indigestible carbohydrates, including resistant starch (RS), which are substrates of the GM. The aim of this study was to evaluate the effect of the indigestible fraction of legumes on the fecal microbiota of normal-weight (NW) and obese (O) donors. Accordingly, a preclinical <i>in vitro</i> fermentation model was developed (Goñi and Martín-Carrón, Nutr Res 18:1077–1089, <CitationRef CitationID="CR10">1998</CitationRef>). Short-chain fatty acid (SCFAs) production was measured via gas chromatography. In addition, the fecal microbiota was characterized via 16&#xa0;S rRNA sequence analysis. The results revealed that the ratio of the relative abundance of Firmicutes to Bacteroidetes was lower in O individuals than in NW individuals. <i>Bacteroides</i> was the predominant genus in the fecal inoculum of the O group. Total SCFAs production was significantly greater in the chickpea (C) group than in the lentil (L) and white bean (WB) groups among the samples from the NW group. In contrast, WB presented the highest production of SCFAs in the samples from the O group. These results suggest that fermentation products (SCFAs) are determined by the components of the legumes, including RS, and the type of microbiota donor (NW or O individuals).</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Effect of the Starchy Legume Source on the In Vitro Fermentation of the Fecal Microbiota from Normal-Weight and Obese Individuals

  • 期刊论文

  • 发表日期：

    2025年1月13日

查看全文
  • Vareska L. Zárate-Córdova,
  • Mónica Sánchez-Tapia,
  • Nimbe Torres,
  • Perla Osorio-Díaz

摘要

The relationship between the gut microbiota (GM) and the health of human beings has been a topic of growing interest in the last few years. Legumes are a rich source of indigestible carbohydrates, including resistant starch (RS), which are substrates of the GM. The aim of this study was to evaluate the effect of the indigestible fraction of legumes on the fecal microbiota of normal-weight (NW) and obese (O) donors. Accordingly, a preclinical in vitro fermentation model was developed (Goñi and Martín-Carrón, Nutr Res 18:1077–1089, 1998). Short-chain fatty acid (SCFAs) production was measured via gas chromatography. In addition, the fecal microbiota was characterized via 16 S rRNA sequence analysis. The results revealed that the ratio of the relative abundance of Firmicutes to Bacteroidetes was lower in O individuals than in NW individuals. Bacteroides was the predominant genus in the fecal inoculum of the O group. Total SCFAs production was significantly greater in the chickpea (C) group than in the lentil (L) and white bean (WB) groups among the samples from the NW group. In contrast, WB presented the highest production of SCFAs in the samples from the O group. These results suggest that fermentation products (SCFAs) are determined by the components of the legumes, including RS, and the type of microbiota donor (NW or O individuals).