Comparative Transcriptome Analyses Provide Potential Insights into Molecular Mechanisms of Anthocyanin-Rich Blueberry Extract in Rapid Intervention Against Acute Alcohol Exposure in Mice

Alcohol-induced health damage has become an increasing global public health concern. Anthocyanins exhibit essential biological activities, including antioxidation, anti-inflammation, and lipid reduction. This study investigates the rapid intervention effects and mechanisms of anthocyanin-rich blueberry extract in mitigating acute alcohol exposure in mice, aiming to uncover its novel nutritional roles. Eight-week-old male C57BL/6J mice were fasted for 6 h and randomly assigned to three groups (CON, EOH, and EOH-BE, n = 8) for the experimental study. The results demonstrated that 1 h after alcohol exposure, BE significantly enhanced the behavioral performance of mice, lowered blood ethanol levels and liver function markers, and alleviated hepatic pathological alterations. GSEA results of KEGG pathways indicated that BE primarily affected pathways associated with nutrient digestion and absorption, energy substance metabolism, unsaturated fatty acids biosynthesis, and gastric cancer, facilitating rapid intervention in acute alcohol exposure in mice. These findings confirm that anthocyanin-rich blueberry extract effectively mitigates the health risks linked to acute alcohol exposure, providing new insights into early intervention and management strategies for alcohol-induced disorders.