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<p><i>Stevia rebaudiana</i> is a plant native to South America known for producing steviol glycosides and fructans used in low-calorie and functional foods. This study aimed to cultivate and isolate inulin from hydroponically grown <i>S. rebaudiana</i> roots. Seedlings were grown hydroponically for 28 days; then, roots were dried and extracted at 121&#xa0;°C for 20&#xa0;min. Extracts were centrifuged and lyophilized, yielding supernatant (SSR-H) and precipitate (PSR-H) fractions. Fructan yields were 21.34% (SSR-H) and 2.91% (PSR-H). HPLC, FTIR, and NMR analyses confirmed inulin-like fructans with polymerization degrees of 16 and 31, respectively. The characteristic peaks of a fructan-like-inulin were observed in the FTIR, ¹H, and ¹³C NMR spectra. These results demonstrate effective inulin extraction from hydroponically grown <i>S. rebaudiana</i> roots, highlighting its potential applications in the food industry.</p>

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Extraction and Characterization of Inulin-Like Fructans from Hydroponically Grown Stevia rebaudiana Roots for Food Applications

  • 期刊论文

  • 发表日期：

    2025年1月14日

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  • Hevelyn Regina da Silva Lima,
  • Gabriela Lafayne Okonski dos Santos,
  • Susana Tavares Cotrim Ribeiro,
  • Maria Amélia Gonçalves,
  • Aline Savam,
  • Regina Aparecida Correia Gonçalves,
  • Arildo José Braz de Oliveira

摘要

Stevia rebaudiana is a plant native to South America known for producing steviol glycosides and fructans used in low-calorie and functional foods. This study aimed to cultivate and isolate inulin from hydroponically grown S. rebaudiana roots. Seedlings were grown hydroponically for 28 days; then, roots were dried and extracted at 121 °C for 20 min. Extracts were centrifuged and lyophilized, yielding supernatant (SSR-H) and precipitate (PSR-H) fractions. Fructan yields were 21.34% (SSR-H) and 2.91% (PSR-H). HPLC, FTIR, and NMR analyses confirmed inulin-like fructans with polymerization degrees of 16 and 31, respectively. The characteristic peaks of a fructan-like-inulin were observed in the FTIR, ¹H, and ¹³C NMR spectra. These results demonstrate effective inulin extraction from hydroponically grown S. rebaudiana roots, highlighting its potential applications in the food industry.