Scholarlink

<p>The generation of voltage (ΔV) by purified photosynthetic protein-pigment complex of photosystem I (PS I) from the cyanobacterium <i>Synechocystis</i> sp. PCC 6803 immobilized onto a porous Millipore nitrocellulose membrane filter (MF) and contacting with indium tin oxide (ITO) semiconductor glass slides, termed ITO|PS I – MF|ITO have been directly measured. In the presence of the ascorbate/2,6-dichlorophenolindophenol redox couple and disaccharide trehalose, photoinduced generation of ΔV with a maximum amplitude was observed upon light irradiation for more than 1&#xa0;h. After storing the ITO|PS I – MF|ITO system in a desiccator at 22 ± 1&#xa0;°C and ~ 65% relative humidity for 7 days in the dark, the photovoltage amplitude decreased by ~ 15%. A significant drop in the ΔV amplitude was observed on the 14th day of storage under these conditions. The amplitude of the stable ΔV signal was fully restored after re-injecting fresh buffer directly into the system, although maximum amplitude was reached more slowly. It is clear that longer preservation of PS I functional activity during storage of the system can be achieved at a higher relative humidity in the desiccator, since the photovoltage was completely suppressed within 24&#xa0;h when the system was stored in the dark in the laboratory room. The protective role of trehalose is also discussed.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Photosystem I: towards stable photovoltage generation in a semi-artificial system

  • 期刊论文

  • 发表日期：

    2025年12月2日

查看全文
  • Mahir Mamedov,
  • Liya Vitukhnovskaya,
  • Andrey Zaspa,
  • Aida Mamedova,
  • Alexey Semenov

摘要

The generation of voltage (ΔV) by purified photosynthetic protein-pigment complex of photosystem I (PS I) from the cyanobacterium Synechocystis sp. PCC 6803 immobilized onto a porous Millipore nitrocellulose membrane filter (MF) and contacting with indium tin oxide (ITO) semiconductor glass slides, termed ITO|PS I – MF|ITO have been directly measured. In the presence of the ascorbate/2,6-dichlorophenolindophenol redox couple and disaccharide trehalose, photoinduced generation of ΔV with a maximum amplitude was observed upon light irradiation for more than 1 h. After storing the ITO|PS I – MF|ITO system in a desiccator at 22 ± 1 °C and ~ 65% relative humidity for 7 days in the dark, the photovoltage amplitude decreased by ~ 15%. A significant drop in the ΔV amplitude was observed on the 14th day of storage under these conditions. The amplitude of the stable ΔV signal was fully restored after re-injecting fresh buffer directly into the system, although maximum amplitude was reached more slowly. It is clear that longer preservation of PS I functional activity during storage of the system can be achieved at a higher relative humidity in the desiccator, since the photovoltage was completely suppressed within 24 h when the system was stored in the dark in the laboratory room. The protective role of trehalose is also discussed.