Scholarlink

<p>Sea barley (<i>Hordeum marinum</i> ssp. <i>marinum</i>) is a wild Triticeae species with notable tolerance to salinity and waterlogging, yet its genetic resources remain poorly characterized. In this study, we assessed the transferability of 111 wheat and barley-derived simple sequence repeats (SSR) markers to <i>H. marinum</i> and evaluated genetic diversity and population structure across ten natural Tunisian populations (150 lines). A total of 38 primers (34.23%) showed successful cross-species transferability in sea barley, of which 13 revealed reproducible polymorphic amplification. These markers detected 45 alleles and revealed substantial variation in gene diversity among populations. The Tabarka population exhibited the highest diversity and the greatest number of private alleles, indicating its conservation relevance. AMOVA showed that most genetic variation resides within populations, and clustering analyses (STRUCTURE, UPGMA, PCoA) revealed genetic structure independent of geographic distance or altitude. This study represents one of the first comprehensive SSR-based assessments of genetic diversity in <i>Hordeum marinum</i>. Findings provide a framework for the conservation and genetic characterization of <i>H. marinum</i> and demonstrate the utility of transferable SSR markers in this species.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The Genetic Diversity of Tunisian Sea Barley (Hordeum marinum ssp. marinum): Insights from Cross-species SSRs

  • 期刊论文

  • 发表日期：

    2026年5月22日

查看全文
  • Warda Saoudi,
  • Wael Taamalli,
  • Mounawer Badri,
  • António Martin,
  • Chedly Abdelly

摘要

Sea barley (Hordeum marinum ssp. marinum) is a wild Triticeae species with notable tolerance to salinity and waterlogging, yet its genetic resources remain poorly characterized. In this study, we assessed the transferability of 111 wheat and barley-derived simple sequence repeats (SSR) markers to H. marinum and evaluated genetic diversity and population structure across ten natural Tunisian populations (150 lines). A total of 38 primers (34.23%) showed successful cross-species transferability in sea barley, of which 13 revealed reproducible polymorphic amplification. These markers detected 45 alleles and revealed substantial variation in gene diversity among populations. The Tabarka population exhibited the highest diversity and the greatest number of private alleles, indicating its conservation relevance. AMOVA showed that most genetic variation resides within populations, and clustering analyses (STRUCTURE, UPGMA, PCoA) revealed genetic structure independent of geographic distance or altitude. This study represents one of the first comprehensive SSR-based assessments of genetic diversity in Hordeum marinum. Findings provide a framework for the conservation and genetic characterization of H. marinum and demonstrate the utility of transferable SSR markers in this species.