Scholarlink

<p>The nuclear factor YA (NF-YA) gene family comprises a class of transcription factors that are widely distributed among eukaryotes and play crucial roles in various biological processes. However, the members of the NF-YA gene family in pear (<i>Pyrus</i> spp.) remain largely uncharacterized, and their functions have not been elucidated. The current study identified a total of 12 NF-YA-related genes from both the wild pear (<i>P. betulifolia</i>) and Chinese white pear (<i>P. bretschneideri</i>). Their protein properties, phylogenetic relationships, motif composition, gene structures, genome synteny, and <i>cis-</i>acting elements were characterized. Furthermore, analysis of the expression patterns of <i>PbNF-YA</i> genes indicated that <i>PbNF-YA3</i>, <i>PbNF-YA4-like</i>, and <i>PbNF-YA6</i> were predominantly expressed in wild pear seeds, with their expression significantly decreased during the progression of dormancy release. Dual-luciferase and yeast one-hybrid assays demonstrated that <i>PbNF-YA4-like</i> represses <i>PbPP2C2</i> promoter activity and participates in ABA-mediated seed dormancy release, although PbNF-YA4-like alone did not bind the promoter in yeast. This study elucidates the structure and evolutionary relationships of the NF-YA gene family in pear and highlights the novel function of <i>PbNF-YA4-like</i> in regulating seed dormancy release in wild pear.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genome-Wide Analysis of NF-YA Gene Family and Seed Dormancy Release Regulation of PbNF-YA4-like in Pear

  • 期刊论文

  • 发表日期：

    2026年5月9日

查看全文
  • Jing Zhang,
  • Wen-Can Miao,
  • Li-hua Zhai,
  • Jia-yi Qian,
  • Xiao Liu,
  • Chun-lei Wang

摘要

The nuclear factor YA (NF-YA) gene family comprises a class of transcription factors that are widely distributed among eukaryotes and play crucial roles in various biological processes. However, the members of the NF-YA gene family in pear (Pyrus spp.) remain largely uncharacterized, and their functions have not been elucidated. The current study identified a total of 12 NF-YA-related genes from both the wild pear (P. betulifolia) and Chinese white pear (P. bretschneideri). Their protein properties, phylogenetic relationships, motif composition, gene structures, genome synteny, and cis-acting elements were characterized. Furthermore, analysis of the expression patterns of PbNF-YA genes indicated that PbNF-YA3, PbNF-YA4-like, and PbNF-YA6 were predominantly expressed in wild pear seeds, with their expression significantly decreased during the progression of dormancy release. Dual-luciferase and yeast one-hybrid assays demonstrated that PbNF-YA4-like represses PbPP2C2 promoter activity and participates in ABA-mediated seed dormancy release, although PbNF-YA4-like alone did not bind the promoter in yeast. This study elucidates the structure and evolutionary relationships of the NF-YA gene family in pear and highlights the novel function of PbNF-YA4-like in regulating seed dormancy release in wild pear.