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<p>Guava is the third richest source of ascorbic acid, an excellent antioxidant. Four guava genotypes namely, Lucknow-49, Shweta, Pant Prabhat, and Arka Kiran were studied concerning ascorbic acid accumulation at 35, 65, 95, and 115&#xa0;days after fruit set during rainy and winter seasons. Two-way factorial Analysis of Variance (ANOVA) design with four genotypes as factor 1 and four stages as factor 2, was performed in four replications for the rainy and winter season at 5&#xa0;% (p &lt; 0.05) significance level. During both the rainy and winter seasons, at 115&#xa0;days after fruit set, Lucknow-49 (82.25 and 166.25&#xa0;mg/ 100&#xa0;g) had higher ascorbic acid content; Shweta (70 and 126&#xa0;mg/ 100&#xa0;g) and Pant Prabhat (52.5 and 112&#xa0;mg/ 100&#xa0;g) had intermediate ascorbic acid content and Arka Kiran (45.5 and 99.75&#xa0;mg/ 100&#xa0;g) had the lowest ascorbic acid content. RT-qPCR was performed to assess the transcript abundance of ascorbic acid pathway genes at 35 and 65 days after fruit set&#xa0;(DAFS), since they showed clear and consistent accumulation of ascorbic acid across the four genotypes. Among the nine genes of the ascorbic acid pathway (L-galactose pathway), two genes, GDP-mannose 3,5-epimerase (<i>GME</i>) and galactose phosphatase (<i>GPP</i>), did not show any detectable expression. Out of the remaining seven genes, the high ascorbic acid containing genotype, L-49 showed detectable and higher expression for all of them; while Arka Kiran, the lowest ascorbic acid containing genotype, showed the least expression. The expression levels were higher at 65 DAFS than 35 DAFS. The downstream genes of ascorbic acid pathway, GDP-L-galactose phosphorylase (<i>GGP),</i> L-galactose dehydrogenase (<i>L-GalDH),</i> and L-galactono-1,4-lactone dehydrogenase (<i>GLDH</i>) were found to be key genes for screening guava genotypes for ascorbic acid content, as their expression patterns were in line with the ascorbic acid content.</p>

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GDP-L-Galactose Phosphorylase (GGP), L-Galactose Dehydrogenase (L-GalDH), and L-Galactono-1,4-Lactone Dehydrogenase (GLDH) are Key Genes for Screening Guava Genotypes for Higher Ascorbic Acid Content

  • 期刊论文

  • 发表日期：

    2026年5月16日

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  • Chukkamettu Anusha,
  • Arumugam Nagaraja,
  • Madhubala Thakre,
  • Poonam Maurya,
  • Shubham Jagga,
  • Eldho Varghese,
  • Nayan Deepak Gangappa,
  • Manish Srivastav,
  • Mohammad Tasleem,
  • Amitha Mithra Sevanthi

摘要

Guava is the third richest source of ascorbic acid, an excellent antioxidant. Four guava genotypes namely, Lucknow-49, Shweta, Pant Prabhat, and Arka Kiran were studied concerning ascorbic acid accumulation at 35, 65, 95, and 115 days after fruit set during rainy and winter seasons. Two-way factorial Analysis of Variance (ANOVA) design with four genotypes as factor 1 and four stages as factor 2, was performed in four replications for the rainy and winter season at 5 % (p < 0.05) significance level. During both the rainy and winter seasons, at 115 days after fruit set, Lucknow-49 (82.25 and 166.25 mg/ 100 g) had higher ascorbic acid content; Shweta (70 and 126 mg/ 100 g) and Pant Prabhat (52.5 and 112 mg/ 100 g) had intermediate ascorbic acid content and Arka Kiran (45.5 and 99.75 mg/ 100 g) had the lowest ascorbic acid content. RT-qPCR was performed to assess the transcript abundance of ascorbic acid pathway genes at 35 and 65 days after fruit set (DAFS), since they showed clear and consistent accumulation of ascorbic acid across the four genotypes. Among the nine genes of the ascorbic acid pathway (L-galactose pathway), two genes, GDP-mannose 3,5-epimerase (GME) and galactose phosphatase (GPP), did not show any detectable expression. Out of the remaining seven genes, the high ascorbic acid containing genotype, L-49 showed detectable and higher expression for all of them; while Arka Kiran, the lowest ascorbic acid containing genotype, showed the least expression. The expression levels were higher at 65 DAFS than 35 DAFS. The downstream genes of ascorbic acid pathway, GDP-L-galactose phosphorylase (GGP), L-galactose dehydrogenase (L-GalDH), and L-galactono-1,4-lactone dehydrogenase (GLDH) were found to be key genes for screening guava genotypes for ascorbic acid content, as their expression patterns were in line with the ascorbic acid content.