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<p>Seasonal changes in environmental conditions regulate the occurrence and severity of abiotic stresses, thereby influencing plant physiology and metabolism. The liverwort <i>Plagiochasma appendiculatum</i>, growing in its natural habitat in Shillong, Meghalaya, India, experiences four ecologically distinct growing seasons: pre-monsoon (GS1), monsoon (GS2), post-monsoon (GS3), and fruiting (GS4). To elucidate the molecular basis of seasonal acclimation of <i>P. appendiculatum</i>, biochemical and proteomic analyses were conducted across these seasons over 3 consecutive years (2019–2021). Biochemical assays revealed significant seasonal variations in oxidative stress markers and pigment content. Levels of malondialdehyde (MDA) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) increased from GS1 to GS4, indicating elevated oxidative stress during later seasons, whereas chlorophyll content peaked in GS2, reflecting favourable photosynthetic conditions. Cytosolic proteome profiling using two-dimensional gel electrophoresis (2-DE) identified 37 differentially abundant protein (DAP) spots corresponding to 27 proteins involved in stress defence, photosynthesis, energy metabolism, growth, and other cellular processes. Stress-related proteins were more abundant during GS3 and GS4, while proteins associated with photosynthesis and energy metabolism were enriched in GS2. Growth- and development-related proteins displayed season-specific abundance patterns reflecting developmental transitions. Enzymatic validation further showed that superoxide dismutase (SOD) activity paralleled its increased protein abundance, confirming its role in reactive oxygen species detoxification during seasonal changes. Pearson’s correlation analysis indicated that protein accumulation was positively associated with humidity but negatively associated with photoperiod. Overall, this study provides insights into the molecular mechanisms underlying seasonal acclimation in <i>P. appendiculatum</i> and identifies key proteins as potential targets for future functional studies in bryophyte stress biology.</p>

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Comparative Seasonal Proteome Profiling of the Liverwort Plagiochasma appendiculatum Reveals Molecular Strategies of Acclimation in its Natural Habitat

  • 期刊论文

  • 发表日期：

    2026年5月8日

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  • Vishal Kumar Jha,
  • Akanksha Srivastava,
  • Sandhya Yadav,
  • Suvajit Basu,
  • Subhankar Biswas,
  • Kritika Tripathi,
  • Neha Chaurasia,
  • Sushil Kumar Singh,
  • Vaibhav Srivastava,
  • Yogesh Mishra

摘要

Seasonal changes in environmental conditions regulate the occurrence and severity of abiotic stresses, thereby influencing plant physiology and metabolism. The liverwort Plagiochasma appendiculatum, growing in its natural habitat in Shillong, Meghalaya, India, experiences four ecologically distinct growing seasons: pre-monsoon (GS1), monsoon (GS2), post-monsoon (GS3), and fruiting (GS4). To elucidate the molecular basis of seasonal acclimation of P. appendiculatum, biochemical and proteomic analyses were conducted across these seasons over 3 consecutive years (2019–2021). Biochemical assays revealed significant seasonal variations in oxidative stress markers and pigment content. Levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2) increased from GS1 to GS4, indicating elevated oxidative stress during later seasons, whereas chlorophyll content peaked in GS2, reflecting favourable photosynthetic conditions. Cytosolic proteome profiling using two-dimensional gel electrophoresis (2-DE) identified 37 differentially abundant protein (DAP) spots corresponding to 27 proteins involved in stress defence, photosynthesis, energy metabolism, growth, and other cellular processes. Stress-related proteins were more abundant during GS3 and GS4, while proteins associated with photosynthesis and energy metabolism were enriched in GS2. Growth- and development-related proteins displayed season-specific abundance patterns reflecting developmental transitions. Enzymatic validation further showed that superoxide dismutase (SOD) activity paralleled its increased protein abundance, confirming its role in reactive oxygen species detoxification during seasonal changes. Pearson’s correlation analysis indicated that protein accumulation was positively associated with humidity but negatively associated with photoperiod. Overall, this study provides insights into the molecular mechanisms underlying seasonal acclimation in P. appendiculatum and identifies key proteins as potential targets for future functional studies in bryophyte stress biology.