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<p>The hemicellulase alpha-<span>l</span>-arabinofuranosidase (ABF) is extensively distributed in nature and plays essential roles in processes including plant growth, development, and abiotic stress responses. Members of the <i>ABF</i> gene family were identified in the genome of <i>Achnatherum splendens</i>. To further clarify the functions of the <i>ABF</i> gene family in <i>A. splendens</i> in response to salt and drought stress, <i>ABF</i> family members were identified using bioinformatics techniques, and their gene structures, conserved motifs, chromosomal localization, subcellular localization predictions, and protein physicochemical properties were analyzed. Quantitative real-time PCR (RT-qPCR) was performed to evaluate the expression profiles of these family members under salt and drought stress conditions. In <i>A. splendens</i>, ten <i>ABF</i> family genes were found to be unevenly distributed across seven chromosomes. Analysis of the physicochemical properties revealed that all AsABF proteins were hydrophilic. According to subcellular localization predictions, the majority of AsABF proteins were localized to vacuoles and chloroplasts. The <i>AsABF</i> family can be categorized into two main clades based on phylogenetic analysis, and members of the same clade share similar gene structures and motif distributions. The promoter regions of <i>AsABF</i> genes contained a significant number of hormone-responsive (e.g., ABA, JA, SA) and stress-responsive cis-elements (e.g., MYB-binding and MYC-binding sites). The expression levels of <i>AsABF1</i>, <i>AsABF9</i>, and <i>AsABF10</i> generally increased under drought stress. Under drought stress, the expression levels of <i>AsABF1</i>, <i>AsABF9</i>, and <i>AsABF10</i> showed an overall upward trend. RT-qPCR analysis confirmed two- to threefold elevations in <i>AsABF10</i> transcript levels at 3, 12, and 24&#xa0;h compared with 0&#xa0;h. Prolonged salt stress gradually increased <i>AsABF9</i> expression, which was approximately one- to twofold higher than the 0&#xa0;h level at 9, 12, and 24&#xa0;h. Collectively, bioinformatics analysis and RT-qPCR experiments revealed that the expression of <i>AsABF9</i> was upregulated under drought and salt stress. These observations suggest that the <i>AsABF</i> gene family may be involved in mediating the molecular and physiological responses of <i>A. splendens</i> to abiotic stress.</p>

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Genome-wide Identification and Expression Analysis of the ABF Gene Family in Achnatherum splendens

  • 期刊论文

  • 发表日期：

    2026年4月6日

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  • Shan Yin,
  • Mingsu Chen,
  • Ming Hu,
  • XiaoYing Li,
  • Jingru Wang,
  • Jiahuan Niu,
  • Zhangqi Song,
  • Huan Yang,
  • Hongbing Li,
  • Rong Li

摘要

The hemicellulase alpha-l-arabinofuranosidase (ABF) is extensively distributed in nature and plays essential roles in processes including plant growth, development, and abiotic stress responses. Members of the ABF gene family were identified in the genome of Achnatherum splendens. To further clarify the functions of the ABF gene family in A. splendens in response to salt and drought stress, ABF family members were identified using bioinformatics techniques, and their gene structures, conserved motifs, chromosomal localization, subcellular localization predictions, and protein physicochemical properties were analyzed. Quantitative real-time PCR (RT-qPCR) was performed to evaluate the expression profiles of these family members under salt and drought stress conditions. In A. splendens, ten ABF family genes were found to be unevenly distributed across seven chromosomes. Analysis of the physicochemical properties revealed that all AsABF proteins were hydrophilic. According to subcellular localization predictions, the majority of AsABF proteins were localized to vacuoles and chloroplasts. The AsABF family can be categorized into two main clades based on phylogenetic analysis, and members of the same clade share similar gene structures and motif distributions. The promoter regions of AsABF genes contained a significant number of hormone-responsive (e.g., ABA, JA, SA) and stress-responsive cis-elements (e.g., MYB-binding and MYC-binding sites). The expression levels of AsABF1, AsABF9, and AsABF10 generally increased under drought stress. Under drought stress, the expression levels of AsABF1, AsABF9, and AsABF10 showed an overall upward trend. RT-qPCR analysis confirmed two- to threefold elevations in AsABF10 transcript levels at 3, 12, and 24 h compared with 0 h. Prolonged salt stress gradually increased AsABF9 expression, which was approximately one- to twofold higher than the 0 h level at 9, 12, and 24 h. Collectively, bioinformatics analysis and RT-qPCR experiments revealed that the expression of AsABF9 was upregulated under drought and salt stress. These observations suggest that the AsABF gene family may be involved in mediating the molecular and physiological responses of A. splendens to abiotic stress.