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<p>Abscisic acid (ABA), known as a senescence hormone, plays a significant role in the senescence process of cut flowers. This study aimed to elucidate the molecular mechanisms of the ABA signaling pathway during the senescence of cut <i>Rhododendron</i>. Based on the <i>Rhododendron simsii</i> genome, 28 gene family members associated with ABA signal transduction were identified, and 26 differentially expressed genes were further screened from the <i>R. Hongshanhu</i> transcriptome. Phylogenetic tree analysis showed that the PYL, PP2C-A, and SnRK2 proteins of rhododendron were divided into three, two, and three subfamilies, respectively. The results revealed closer genetic relationships with dicotyledons than with monocotyledons. Conservative motif analysis indicated that PYLs, PP2C-As, SnRK2s, and ABI5/ABF contained 11, 20, 10, and 13 conservative motifs, respectively. Analysis of cis-acting elements reveals that most of the rhododendron gene family possesses abscisic acid response elements, with <i>RsPP2C42</i> having the highest number. Transcriptome analysis showed that 10 genes were upregulated and five genes were downregulated in “Hongshanhu.” Quantitative polymerase chain reaction (qPCR) analysis demonstrated that <i>PYL9</i>, <i>PP2C3</i>, <i>PP2C42</i>, and <i>SnRK2.3</i> were significantly upregulated in the early senescence stages in “Hongshanhu.” Notably, <i>PP2C3</i> was significantly upregulated during stages III and IV in <i>R. simsii.</i> The results suggest that these genes may play a pivotal role in regulating cut flower senescence in <i>Rhododendron</i>. These findings provide important insights into the molecular mechanisms underlying ABA-mediated cut flower senescence.</p>

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Screening and Analysis of Abscisic Acid Signal Transduction Genes in Cut Rhododendron Flowers During Senescence

  • 期刊论文

  • 发表日期：

    2026年3月18日

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  • Na Song,
  • Zhili Jiang,
  • Fanyu Zeng,
  • Xingmin Geng

摘要

Abscisic acid (ABA), known as a senescence hormone, plays a significant role in the senescence process of cut flowers. This study aimed to elucidate the molecular mechanisms of the ABA signaling pathway during the senescence of cut Rhododendron. Based on the Rhododendron simsii genome, 28 gene family members associated with ABA signal transduction were identified, and 26 differentially expressed genes were further screened from the R. Hongshanhu transcriptome. Phylogenetic tree analysis showed that the PYL, PP2C-A, and SnRK2 proteins of rhododendron were divided into three, two, and three subfamilies, respectively. The results revealed closer genetic relationships with dicotyledons than with monocotyledons. Conservative motif analysis indicated that PYLs, PP2C-As, SnRK2s, and ABI5/ABF contained 11, 20, 10, and 13 conservative motifs, respectively. Analysis of cis-acting elements reveals that most of the rhododendron gene family possesses abscisic acid response elements, with RsPP2C42 having the highest number. Transcriptome analysis showed that 10 genes were upregulated and five genes were downregulated in “Hongshanhu.” Quantitative polymerase chain reaction (qPCR) analysis demonstrated that PYL9, PP2C3, PP2C42, and SnRK2.3 were significantly upregulated in the early senescence stages in “Hongshanhu.” Notably, PP2C3 was significantly upregulated during stages III and IV in R. simsii. The results suggest that these genes may play a pivotal role in regulating cut flower senescence in Rhododendron. These findings provide important insights into the molecular mechanisms underlying ABA-mediated cut flower senescence.