Scholarlink

<p>Minor millets are an important food source for millions of people in developing countries across Asia and Africa. Kodo millet is a nutrient-rich crop that is well adapted to marginal lands and can produce high grain yields under harsh environmental conditions. However, crop improvement efforts are constrained by limited genomics resources, resulting in relatively few genetic diversity studies using molecular markers. In this study, we evaluated 15 morphological traits and assessed the cross-transferability of simple sequence repeat (SSR) markers from foxtail millet, pearl millet, and rice in 103 kodo millet genotypes. Principal component analysis revealed that the first five components accounted for 70.13% of the total genetic variation. Plant height, days to 50% flowering, thumb length, and days to first flowering contributed most strongly to the first principal component. Flag leaf blade length, Zn content, and thousand-grain weight were prominent contributors to the second component, whereas basal and productive tillers dominated the third component. Biplot analysis showed positive correlations between flag leaf blade length and Zn content, as well as among plant height, days to first flowering, and days to 50% flowering. Cross-transferability analysis showed that 81.81% of foxtail millet SSRs and 75% of pearl millet markers were transferable to kodo millet, with polymorphism rates of 22.22% and 24.24%, respectively. Polymorphic information content (PIC) values ranged from 0.05 to 0.99, with an average of 0.572. Cluster analysis grouped the 103 genotypes into 21 distinct clusters, highlighting substantial genetic diversity and relationships among the genotypes. Collectively, these informative SSR markers provide valuable resources for kodo millet breeding and genetic improvement programs.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Cross Transferability of Foxtail Millet, Pearl Millet and Rice SSR Markers for Genetic Diversity Analysis in Kodo Millet (Paspalum scrobiculatum)

  • 期刊论文

  • 发表日期：

    2026年2月7日

查看全文
  • Nirubana Varatharajan,
  • Ganesamurthy ‪Kulandaivelu,
  • Ravikesavan Rajasekaran,
  • Chitdeshwari Thiyagarajan,
  • Palaniyappan Subramani,
  • Vigneshwaran Raja,
  • Karthikeyan Adhimoolam

摘要

Minor millets are an important food source for millions of people in developing countries across Asia and Africa. Kodo millet is a nutrient-rich crop that is well adapted to marginal lands and can produce high grain yields under harsh environmental conditions. However, crop improvement efforts are constrained by limited genomics resources, resulting in relatively few genetic diversity studies using molecular markers. In this study, we evaluated 15 morphological traits and assessed the cross-transferability of simple sequence repeat (SSR) markers from foxtail millet, pearl millet, and rice in 103 kodo millet genotypes. Principal component analysis revealed that the first five components accounted for 70.13% of the total genetic variation. Plant height, days to 50% flowering, thumb length, and days to first flowering contributed most strongly to the first principal component. Flag leaf blade length, Zn content, and thousand-grain weight were prominent contributors to the second component, whereas basal and productive tillers dominated the third component. Biplot analysis showed positive correlations between flag leaf blade length and Zn content, as well as among plant height, days to first flowering, and days to 50% flowering. Cross-transferability analysis showed that 81.81% of foxtail millet SSRs and 75% of pearl millet markers were transferable to kodo millet, with polymorphism rates of 22.22% and 24.24%, respectively. Polymorphic information content (PIC) values ranged from 0.05 to 0.99, with an average of 0.572. Cluster analysis grouped the 103 genotypes into 21 distinct clusters, highlighting substantial genetic diversity and relationships among the genotypes. Collectively, these informative SSR markers provide valuable resources for kodo millet breeding and genetic improvement programs.