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<p>Ellagic acid (EA) seed priming offers a novel, sustainable approach to enhance barley (<i>Hordeum vulgare</i> L.) tolerance to ultraviolet-B (UV-B) stress, a growing constraint on crop productivity in many cereal-growing regions. Therefore, genome-wide SNP mapping uncovers ellagic acid–specific QTLs shaping oxidative defense and early growth of barley under UV stress. This study’s diverse collection of 138 barley genotypes was primed with 50 mg L⁻<sup>1</sup> EA before germination under controlled UV-B exposure. Comparative assays of germination rate, seedling vigor, seedling biomass, and chlorophyll content demonstrated that EA-primed seedlings maintained significantly higher performance metrics than unprimed controls. Biochemical analyses revealed a marked reduction in reactive oxygen species (ROS) accumulation and lipid peroxidation, alongside enhanced activities of superoxide dismutase, catalase, ascorbate peroxidase, and malondialdehyde (MDA) levels. Elevated levels of phenolic antioxidants in EA-primed seedlings indicate reinforced redox buffering capacity. Leveraging a diverse barley germplasm panel genotyped with a high-density single-nucleotide polymorphism (SNP) array, we conducted a genome-wide association study (GWAS) to map quantitative trait loci (QTL) linked to EA-mediated UV-B tolerance and antioxidant metabolite accumulation. Several loci on chromosomes 2H and 5H co-localized with candidate genes involved in polyphenol transport, ROS detoxification, and UV-B signal transduction. Our integrative framework, merging biochemical priming, metabolite profiling, and GWAS, delivers SNP markers tagging key antioxidant-enzyme and chlorophyll-stability QTLs, enabling marker-assisted breeding of barley cultivars with priming-boosted UV-B resilience.</p>

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Ellagic Acid Seed Priming Reconfigures Polygenic Networks to Harmonize Antioxidant Defense and Growth in Barley Under UV-B Radiation: Insights from Genome-Wide Association Study

  • 期刊论文

  • 发表日期：

    2026年2月19日

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  • Khairiah Mubarak Alwutayd,
  • Ashwag Shami,
  • Ahmad M. Alqudah,
  • Samar G. Thabet

摘要

Ellagic acid (EA) seed priming offers a novel, sustainable approach to enhance barley (Hordeum vulgare L.) tolerance to ultraviolet-B (UV-B) stress, a growing constraint on crop productivity in many cereal-growing regions. Therefore, genome-wide SNP mapping uncovers ellagic acid–specific QTLs shaping oxidative defense and early growth of barley under UV stress. This study’s diverse collection of 138 barley genotypes was primed with 50 mg L⁻1 EA before germination under controlled UV-B exposure. Comparative assays of germination rate, seedling vigor, seedling biomass, and chlorophyll content demonstrated that EA-primed seedlings maintained significantly higher performance metrics than unprimed controls. Biochemical analyses revealed a marked reduction in reactive oxygen species (ROS) accumulation and lipid peroxidation, alongside enhanced activities of superoxide dismutase, catalase, ascorbate peroxidase, and malondialdehyde (MDA) levels. Elevated levels of phenolic antioxidants in EA-primed seedlings indicate reinforced redox buffering capacity. Leveraging a diverse barley germplasm panel genotyped with a high-density single-nucleotide polymorphism (SNP) array, we conducted a genome-wide association study (GWAS) to map quantitative trait loci (QTL) linked to EA-mediated UV-B tolerance and antioxidant metabolite accumulation. Several loci on chromosomes 2H and 5H co-localized with candidate genes involved in polyphenol transport, ROS detoxification, and UV-B signal transduction. Our integrative framework, merging biochemical priming, metabolite profiling, and GWAS, delivers SNP markers tagging key antioxidant-enzyme and chlorophyll-stability QTLs, enabling marker-assisted breeding of barley cultivars with priming-boosted UV-B resilience.