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<p>Alfalfa (<i>Medicago sativa</i> L.) is a widely grown crop valued for its high yield, nutritional value, and adaptability. However, salt stress can reduce its growth and yield. Gibberellin (GA), a plant hormone, can improve alfalfa’s salt tolerance by adjusting ion balance, osmotic pressure, and antioxidant activity. Despite this, the detailed genetic and molecular processes of how GA helps alfalfa tolerate salt stress are not fully understood. It is found that salt stress reduced germination, root length, and weight, but GA<sub>3</sub>, especially at 10&#xa0;μM, could coordinate the balance between plant growth and salt stress, playing an important role in protecting plants from salt stress. Research has shown that under salt stress, supplementing with 10&#xa0;μM GA<sub>3</sub> increases superoxide anion (O<sub>2</sub><sup>−</sup>) levels and enhances the activities of superoxide dismutase (SOD) and peroxidase (POD), thereby boosting the antioxidant capacity of alfalfa. Reduced levels of Glutathione (GSH) due to salt stress were also restored by GA<sub>3</sub>. Transcriptomic analysis showed 4,206 genes were differently expressed under salt stress, with 2,088 up- and 2,118 down-regulated. With GA<sub>3</sub> treatment, 5,352 genes were differently expressed, 2,706 up- and 2,646 down-regulated. The addition of GA<sub>3</sub> to salt stress treatment resulted in 282 differently expressed genes, 129 up- and 153 down-regulated. The genes were mainly involved in the biosynthesis of flavonoids, plant hormones, glutathione, soluble sugars, and related to ATP-binding cassette (ABC) transporters and mitogen-activated protein kinase (MAPK) signaling pathways. This indicates that GA<sub>3</sub>’s salt tolerance mechanism is linked to energy supply and antioxidant gene regulation, which could be useful for developing salt-tolerant crops.</p>

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Identification of Gibberellin-mediated Key Genes for Salt Tolerance in Alfalfa Based on Transcriptomic Analysis

  • 期刊论文

  • 发表日期：

    2026年1月21日

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  • Guomin Li,
  • Xiangling Ren,
  • Wenxuan Zhu,
  • Yingao Li,
  • Zirui Liu,
  • Xi Wang,
  • Defeng Li,
  • Yinghua Shi,
  • Xiaoyan Zhu,
  • Chengzhang Wang,
  • Hao Sun

摘要

Alfalfa (Medicago sativa L.) is a widely grown crop valued for its high yield, nutritional value, and adaptability. However, salt stress can reduce its growth and yield. Gibberellin (GA), a plant hormone, can improve alfalfa’s salt tolerance by adjusting ion balance, osmotic pressure, and antioxidant activity. Despite this, the detailed genetic and molecular processes of how GA helps alfalfa tolerate salt stress are not fully understood. It is found that salt stress reduced germination, root length, and weight, but GA3, especially at 10 μM, could coordinate the balance between plant growth and salt stress, playing an important role in protecting plants from salt stress. Research has shown that under salt stress, supplementing with 10 μM GA3 increases superoxide anion (O2−) levels and enhances the activities of superoxide dismutase (SOD) and peroxidase (POD), thereby boosting the antioxidant capacity of alfalfa. Reduced levels of Glutathione (GSH) due to salt stress were also restored by GA3. Transcriptomic analysis showed 4,206 genes were differently expressed under salt stress, with 2,088 up- and 2,118 down-regulated. With GA3 treatment, 5,352 genes were differently expressed, 2,706 up- and 2,646 down-regulated. The addition of GA3 to salt stress treatment resulted in 282 differently expressed genes, 129 up- and 153 down-regulated. The genes were mainly involved in the biosynthesis of flavonoids, plant hormones, glutathione, soluble sugars, and related to ATP-binding cassette (ABC) transporters and mitogen-activated protein kinase (MAPK) signaling pathways. This indicates that GA3’s salt tolerance mechanism is linked to energy supply and antioxidant gene regulation, which could be useful for developing salt-tolerant crops.