<p>Maize is a highly versatile crop, having the highest productivity among the cereals; however, its genetic diversity is declining day by day. Hence, the use of wild relatives is an important approach to enhance the climate resiliency in maize. This study aimed to develop a genetic mapping population (BC<sub>2</sub>F<sub>2</sub>) by crossing the maize inbred line LM 13 with <i>Zea mays</i> ssp. <i>parviglumis</i>. Further, genomic regions/quantitative trait loci (QTLs) were identified for seedling-related traits at BC<sub>2</sub>F<sub>3</sub> families. The phenotypic traits, SPAD value, plant height, number of leaves, root length, fresh shoot weight, dry shoot weight, fresh root weight and dry root weight showed significant variation in BC<sub>2</sub>F<sub>3</sub> families. These phenotypic traits showed 21 significant correlations among each other which can help in the simultaneous improvement of traits. A total of 102 SSR markers spanning across all 10 chromosomes were used for linkage map construction. A total of five QTLs were identified, one each for root length (chromosome 1) and fresh root weight (chromosome 9) and three for fresh shoot weight on chromosomes 2 and 4. The QTLs for root length (<i>qRL1.1</i>) and shoot fresh weight (<i>qFSW2.1</i>) were contributed by <i>Zea mays</i> ssp. <i>parviglumis.</i> These identified QTLs from <i>Zea mays</i> ssp. <i>parviglumis</i> may be targeted for developing climate-resilient maize cultivars.</p>

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Exploring Teosinte (Zea mays ssp. parviglumis) for Identification of Genomic Regions for Root and Shoot Traits at Seedling Stage in Maize

  • Pardeep Kumar,
  • Mukesh Choudhary,
  • Bhupender Kumar,
  • Ningthai Longmei,
  • Bahadur Singh Jat,
  • Ankush Sharma

摘要

Maize is a highly versatile crop, having the highest productivity among the cereals; however, its genetic diversity is declining day by day. Hence, the use of wild relatives is an important approach to enhance the climate resiliency in maize. This study aimed to develop a genetic mapping population (BC2F2) by crossing the maize inbred line LM 13 with Zea mays ssp. parviglumis. Further, genomic regions/quantitative trait loci (QTLs) were identified for seedling-related traits at BC2F3 families. The phenotypic traits, SPAD value, plant height, number of leaves, root length, fresh shoot weight, dry shoot weight, fresh root weight and dry root weight showed significant variation in BC2F3 families. These phenotypic traits showed 21 significant correlations among each other which can help in the simultaneous improvement of traits. A total of 102 SSR markers spanning across all 10 chromosomes were used for linkage map construction. A total of five QTLs were identified, one each for root length (chromosome 1) and fresh root weight (chromosome 9) and three for fresh shoot weight on chromosomes 2 and 4. The QTLs for root length (qRL1.1) and shoot fresh weight (qFSW2.1) were contributed by Zea mays ssp. parviglumis. These identified QTLs from Zea mays ssp. parviglumis may be targeted for developing climate-resilient maize cultivars.