Scholarlink

Abstract <p>Lipoxygenases (LOX) are non-heme, non-sulfur iron-containing enzymes involved in lipid oxidation, playing key roles in plant development and stress responses. Despite their functional significance, <i>LOX</i> genes remain poorly characterized in climate-resilient crops like pearl millet (<i>Pennisetum glaucum</i>), which is prone to grain rancidity—a major post-harvest problem that affects quality and shelf life. In this study, ten <i>PgLOX</i> genes were identified and classified into 9-LOX and 13-LOX subfamilies, with uneven distribution across chromosomes and evidence of tandem and segmental duplications. Evolutionary analysis indicated purifying selection. Motif analysis revealed conserved motifs within the <i>PgLOX</i> gene family. <i>Cis</i>-motif analysis showed that the potential promoter regions of the <i>PgLOX</i> genes contain various elements that are tissue-specific, related to development, stress-responsive, and hormone-responsive. Protein–protein interaction predictions suggested functional connections among eight PgLOX proteins. qRT-PCR analysis showed that among the ten <i>PgLOX</i> genes, <i>PgLOX1</i> was significantly expressed in the grains of genotypes susceptible to rancidity, whereas it was not present in leaf tissues. These findings indicate that <i>PgLOX1</i> may be involved in lipid peroxidation and oxidative rancidity in pearl millet grains, emphasizing its potential for functional validation and gene editing to enhance grain quality.</p> Main Conclusion <p>The <i>Pennisetum glaucum L.</i> gene likely contributes to oxidative rancidity in pearl millet grains and may be&#xa0;a potential candidate&#xa0;for gene editing to improve grain quality and extend&#xa0;shelf life.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genome-Wide Identification and Expression Analysis of Lipoxygenase (LOX) Gene Family in Pearl Millet (Pennisetum Glaucum L.)

  • 期刊论文

  • 发表日期：

    2025年11月6日

查看全文
  • Sudhakar Reddy Palakolanu,
  • Pradeep Reddy Bommineni,
  • Wricha Tyagi

摘要

Abstract

Lipoxygenases (LOX) are non-heme, non-sulfur iron-containing enzymes involved in lipid oxidation, playing key roles in plant development and stress responses. Despite their functional significance, LOX genes remain poorly characterized in climate-resilient crops like pearl millet (Pennisetum glaucum), which is prone to grain rancidity—a major post-harvest problem that affects quality and shelf life. In this study, ten PgLOX genes were identified and classified into 9-LOX and 13-LOX subfamilies, with uneven distribution across chromosomes and evidence of tandem and segmental duplications. Evolutionary analysis indicated purifying selection. Motif analysis revealed conserved motifs within the PgLOX gene family. Cis-motif analysis showed that the potential promoter regions of the PgLOX genes contain various elements that are tissue-specific, related to development, stress-responsive, and hormone-responsive. Protein–protein interaction predictions suggested functional connections among eight PgLOX proteins. qRT-PCR analysis showed that among the ten PgLOX genes, PgLOX1 was significantly expressed in the grains of genotypes susceptible to rancidity, whereas it was not present in leaf tissues. These findings indicate that PgLOX1 may be involved in lipid peroxidation and oxidative rancidity in pearl millet grains, emphasizing its potential for functional validation and gene editing to enhance grain quality.

Main Conclusion

The Pennisetum glaucum L. gene likely contributes to oxidative rancidity in pearl millet grains and may be a potential candidate for gene editing to improve grain quality and extend shelf life.