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<p>Garden pea (<i>Pisum sativum</i> L., 2n = 2x = 14) is commonly grown cool season leguminous vegetable in India. The knowledge on crop genetic diversity is essential for its exploitation in breeding programmes. The present study was carried out to assess morphological and molecular diversity among 82 garden pea genotypes. The genotypes were characterized for 9 qualitative and 11 quantitative morphological parameters during two cropping seasons of 2020–2021 and 2021–2022. The genetic analysis revealed wide variation among all the lines under study for all the agro-morphological traits. The cluster analysis and heat map based on quantitative morphological traits classified most of the exotic genotypes together. Similarly, molecular diversity estimated using 38 polymorphic SSR markers revealed that the major allele frequency ranged from 0.23 to 0.95 and allele number ranged from 2 to 10 with an average of 5.00 alleles per marker. Among 38 polymorphic SSR markers, 32 markers had PIC value more than 0.3 with mean PIC of 0.46. The NJ dendrogram classified the 82 genotypes into 2 major clusters. The population structure analysis revealed that there were four distinct populations in studied garden pea genotypes and it was observed that similar to morphological clustering, population structure analysis also grouped most of the exotic genotypes together in population IV. Analysis of molecular variance (AMOVA) revealed that there was significant molecular difference among the studied genotypes. Thus, a combined approach of morphological and molecular diversity analysis will be helpful in widening the genetic base of garden pea genotypes for future breeding programmes.</p>

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Population Structure and Diversity Analysis Using SSR Markers in Garden Pea (Pisum sativum L.): Implications in Breeding

  • 期刊论文

  • 发表日期：

    2025年7月28日

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  • Janani R.,
  • Brij Bihari Sharma,
  • Shri Dhar,
  • Ajay Arora,
  • Prakash Kumar,
  • Harshawardhan Choudhary,
  • Ramesh Kumar Yadav,
  • Vinod Kumar Sharma,
  • Amolkumar U. Solanke,
  • Dharmendra Singh,
  • Saurabh Singh

摘要

Garden pea (Pisum sativum L., 2n = 2x = 14) is commonly grown cool season leguminous vegetable in India. The knowledge on crop genetic diversity is essential for its exploitation in breeding programmes. The present study was carried out to assess morphological and molecular diversity among 82 garden pea genotypes. The genotypes were characterized for 9 qualitative and 11 quantitative morphological parameters during two cropping seasons of 2020–2021 and 2021–2022. The genetic analysis revealed wide variation among all the lines under study for all the agro-morphological traits. The cluster analysis and heat map based on quantitative morphological traits classified most of the exotic genotypes together. Similarly, molecular diversity estimated using 38 polymorphic SSR markers revealed that the major allele frequency ranged from 0.23 to 0.95 and allele number ranged from 2 to 10 with an average of 5.00 alleles per marker. Among 38 polymorphic SSR markers, 32 markers had PIC value more than 0.3 with mean PIC of 0.46. The NJ dendrogram classified the 82 genotypes into 2 major clusters. The population structure analysis revealed that there were four distinct populations in studied garden pea genotypes and it was observed that similar to morphological clustering, population structure analysis also grouped most of the exotic genotypes together in population IV. Analysis of molecular variance (AMOVA) revealed that there was significant molecular difference among the studied genotypes. Thus, a combined approach of morphological and molecular diversity analysis will be helpful in widening the genetic base of garden pea genotypes for future breeding programmes.