Scholarlink

<p>The study of trihelix transcription factors has become increasingly important as research in molecular biology has been refined and deepened. Trihelix transcription factors are involved in many plant growth and developmental processes. Currently, the functions of trihelix transcription factors make trihelix transcription factors play an important role in many abiotic stresses, especially low temperature, drought, salinity, abscisic acid, methyl jasmonate, and many other abiotic stresses. However, the trihelix gene family has not yet been reported in <i>Polygonatum kingianum</i>. We identified 30 <i>trihelix</i> (<i>GT</i>) gene family members in the <i>P. kingianum</i> transcriptome database. Bioinformatic methods were used to analyze the physical and chemical properties of the <i>trihelix</i> gene family members, GO functional annotations, <i>cis</i>-acting elements, subcellular localization, intranuclear subcellular localization, intraspecific evolutionary relationships, and expression patterns induced by abiotic stress. The results showed that 80% of the <i>trihelix</i> gene family members were located in the nucleus. GO annotation of <i>PkGT</i> genes indicated that they are involved in various biological and molecular functions related to plant growth, development, and stress responses. The promoter region of <i>PkGT</i> genes was found to be involved in a variety of biological and molecular functions related to low temperature, hormone response, light response, growth, and development, and <i>cis</i>-acting regulatory elements were identified in the promoter region of <i>PkGT</i> genes related to various abiotic stresses, such as low temperature, hormone response, light response, growth, and development. Thirty <i>PkGT</i> genes were classified into five families, namely GT-1, GT-2, GTγ, SIPI, and SH4 family subgroups, among which the SIPI family had the highest number of members. Correlation analysis showed that <i>PkGT</i> genes were significantly expressed in the roots, stems, and leaves, with the most significant expression in the roots and stems. The most significant expression was found in the roots, and co-expression network analysis showed that <i>PkGT</i> genes interacted with each other, and most of the genes promoted each other. We also found four <i>PkGT</i> genes in the SIPI subgroup with more regulatory elements responded and were higher expression after MeJA treatment. This preliminary study provides a theoretical basis for the function of <i>GT</i> gene family members in <i>P. kingianum</i>. This also lays the groundwork for investigating their functions in various biological processes and signaling pathways.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genome-Wide Identification and Characterization of the Trihelix Transcription Factor Family in Polygonatum kingianum and Gene Expression Pattern Analysis Under MeJA Treatment

  • 期刊论文

  • 发表日期：

    2025年8月28日

查看全文
  • Chun-Yu Chen,
  • Wan-Bo Ding,
  • Hong-Jing Zhang,
  • Yan-Ran Qi,
  • Wen-Xiang Wang

摘要

The study of trihelix transcription factors has become increasingly important as research in molecular biology has been refined and deepened. Trihelix transcription factors are involved in many plant growth and developmental processes. Currently, the functions of trihelix transcription factors make trihelix transcription factors play an important role in many abiotic stresses, especially low temperature, drought, salinity, abscisic acid, methyl jasmonate, and many other abiotic stresses. However, the trihelix gene family has not yet been reported in Polygonatum kingianum. We identified 30 trihelix (GT) gene family members in the P. kingianum transcriptome database. Bioinformatic methods were used to analyze the physical and chemical properties of the trihelix gene family members, GO functional annotations, cis-acting elements, subcellular localization, intranuclear subcellular localization, intraspecific evolutionary relationships, and expression patterns induced by abiotic stress. The results showed that 80% of the trihelix gene family members were located in the nucleus. GO annotation of PkGT genes indicated that they are involved in various biological and molecular functions related to plant growth, development, and stress responses. The promoter region of PkGT genes was found to be involved in a variety of biological and molecular functions related to low temperature, hormone response, light response, growth, and development, and cis-acting regulatory elements were identified in the promoter region of PkGT genes related to various abiotic stresses, such as low temperature, hormone response, light response, growth, and development. Thirty PkGT genes were classified into five families, namely GT-1, GT-2, GTγ, SIPI, and SH4 family subgroups, among which the SIPI family had the highest number of members. Correlation analysis showed that PkGT genes were significantly expressed in the roots, stems, and leaves, with the most significant expression in the roots and stems. The most significant expression was found in the roots, and co-expression network analysis showed that PkGT genes interacted with each other, and most of the genes promoted each other. We also found four PkGT genes in the SIPI subgroup with more regulatory elements responded and were higher expression after MeJA treatment. This preliminary study provides a theoretical basis for the function of GT gene family members in P. kingianum. This also lays the groundwork for investigating their functions in various biological processes and signaling pathways.