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<p><i>Botrytis cinerea</i> causes a fungal disease that poses a significant economic threat to grape production. To better understand the molecular mechanisms underlying grape resistance to <i>B. cinerea</i>, this study focused on the resistant grape variety “Shuangyou” (SY) and the susceptible variety “Pinot Noir” (PN) as experimental materials. Both varieties were inoculated with <i>B. cinerea</i>, and high-throughput RNA sequencing was employed to analyze their respective transcriptomes at 12&#xa0;h post-infection (hpi). A total of 13,757 differentially expressed genes (DEGs) were identified in both varieties. There were 4519 common DEGs between SY and PN, suggesting their potential relevance to grape resistance to <i>B. cinerea</i>. Furthermore, functional annotation and pathway analysis revealed that the 20 most enriched metabolic pathways among the DEGs included those related to flavonoid biosynthesis, glyceride metabolism, plant hormone signal transduction, MAPK signaling pathway, and plant–pathogen interactions. This indicates that grape resistance to <i>B. cinerea</i> is regulated through multiple pathways rather than a singular mechanism. Among these pathways, those related to MAPK signaling, plant–pathogen interactions, and flavonoid biosynthesis are interconnected with disease resistance. Investigation of genes shared by these pathways revealed 16 DEGs as potential candidates. Validation through qPCR confirmed that the expression trends of these genes were consistent with the transcriptome sequencing results. Moreover, the transient transformation of grape leaves with a candidate gene identified above, <i>VaWRKY24</i>, using <i>Agrobacterium tumefaciens</i>, demonstrated that <i>VaWRKY24</i> enhances grape resistance to <i>B. cinerea</i>. These findings lay the foundation for further research on the interaction between grape and <i>B. cinerea</i> and the identification and utilization of disease-resistant genes, providing insights for the breeding of grape varieties resistant to <i>B. cinerea</i>.</p>

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Transcriptome Analysis Reveals the Response Mechanism of Grape Leaves to Botrytis cinerea

  • 期刊论文

  • 发表日期：

    2025年6月24日

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  • Chengnan Li,
  • Yulei Zhao,
  • Denghui Ma,
  • Rongrong Ma,
  • Jinxuan Zhang,
  • Xiao Yin

摘要

Botrytis cinerea causes a fungal disease that poses a significant economic threat to grape production. To better understand the molecular mechanisms underlying grape resistance to B. cinerea, this study focused on the resistant grape variety “Shuangyou” (SY) and the susceptible variety “Pinot Noir” (PN) as experimental materials. Both varieties were inoculated with B. cinerea, and high-throughput RNA sequencing was employed to analyze their respective transcriptomes at 12 h post-infection (hpi). A total of 13,757 differentially expressed genes (DEGs) were identified in both varieties. There were 4519 common DEGs between SY and PN, suggesting their potential relevance to grape resistance to B. cinerea. Furthermore, functional annotation and pathway analysis revealed that the 20 most enriched metabolic pathways among the DEGs included those related to flavonoid biosynthesis, glyceride metabolism, plant hormone signal transduction, MAPK signaling pathway, and plant–pathogen interactions. This indicates that grape resistance to B. cinerea is regulated through multiple pathways rather than a singular mechanism. Among these pathways, those related to MAPK signaling, plant–pathogen interactions, and flavonoid biosynthesis are interconnected with disease resistance. Investigation of genes shared by these pathways revealed 16 DEGs as potential candidates. Validation through qPCR confirmed that the expression trends of these genes were consistent with the transcriptome sequencing results. Moreover, the transient transformation of grape leaves with a candidate gene identified above, VaWRKY24, using Agrobacterium tumefaciens, demonstrated that VaWRKY24 enhances grape resistance to B. cinerea. These findings lay the foundation for further research on the interaction between grape and B. cinerea and the identification and utilization of disease-resistant genes, providing insights for the breeding of grape varieties resistant to B. cinerea.