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<p>Indian mustard is a major oilseed crop cultivated in south Asia. Hybrid breeding has been successfully employed in this crop with the development of CMS and fertility restorer systems. Parents from diverse gene pools are likely to produce hybrids with high level of heterosis, therefore, selecting parents with suitable diversity are necessary for deploying them in hybrid breeding program(s). In the present study, 88 maintainer lines of Indian mustard developed at different research institutions in India were delineated into diverse gene pools on the basis of morphological traits and SSR (simple sequence repeat) markers. A total of 11 morphological traits and 270 SSRs were used to evaluate diversity among 88 Indian mustard genotypes. Highest variability was recorded for number of secondary branches, followed by seed yield, seed weight, primary branches, and siliquae on main raceme. A total of 255 (94.44%) SSRs produced polymorphic amplicons having 766 alleles and allele number ranged from 2 to 10 with 3.0 mean number of alleles per locus. All the 88 Indian mustard genotypes were grouped into four major clusters on the basis of morphological traits, whereas SSR allelic data grouped them into seven clusters. Population structure analysis divided all the genotypes into two subpopulations and revealed the presence of significant admixture genotypes. Genetically diverse genotypes were identified for use in hybrid breeding program for high heterosis. Information generated through this study will be highly useful in identification of specific combiners and suitable maintainers for conversion to CMS or fertility restorer lines and their deployment in national hybrid mustard breeding program.</p>

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Patterns of Genetic Diversity Among Maintainer Lines of Indian Mustard by Using Morphological and SSR Markers

  • 期刊论文

  • 发表日期：

    2025年5月10日

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  • K. H. Singh,
  • Naveen Singh,
  • Ram Avtar,
  • Gurpreet Kaur,
  • Guman Singh,
  • Nehanjali Parmar,
  • Lal Singh,
  • Chhaya Atri,
  • A. K. Thakur

摘要

Indian mustard is a major oilseed crop cultivated in south Asia. Hybrid breeding has been successfully employed in this crop with the development of CMS and fertility restorer systems. Parents from diverse gene pools are likely to produce hybrids with high level of heterosis, therefore, selecting parents with suitable diversity are necessary for deploying them in hybrid breeding program(s). In the present study, 88 maintainer lines of Indian mustard developed at different research institutions in India were delineated into diverse gene pools on the basis of morphological traits and SSR (simple sequence repeat) markers. A total of 11 morphological traits and 270 SSRs were used to evaluate diversity among 88 Indian mustard genotypes. Highest variability was recorded for number of secondary branches, followed by seed yield, seed weight, primary branches, and siliquae on main raceme. A total of 255 (94.44%) SSRs produced polymorphic amplicons having 766 alleles and allele number ranged from 2 to 10 with 3.0 mean number of alleles per locus. All the 88 Indian mustard genotypes were grouped into four major clusters on the basis of morphological traits, whereas SSR allelic data grouped them into seven clusters. Population structure analysis divided all the genotypes into two subpopulations and revealed the presence of significant admixture genotypes. Genetically diverse genotypes were identified for use in hybrid breeding program for high heterosis. Information generated through this study will be highly useful in identification of specific combiners and suitable maintainers for conversion to CMS or fertility restorer lines and their deployment in national hybrid mustard breeding program.