Scholarlink

<p>Metal tolerance proteins (MTPs) play central roles in metal homeostasis in plant cells and tissues. Cotton (<i>Gossypium hirsutum</i>) has strong tolerance to metal stresses and can be used as a phytoremediation crop for heavy metal-contaminated areas. However, the information and biological functions of the MTP gene family in cotton and their roles in metal stresses remain unclear. In this study, a comprehensive analysis of MTP genes was carried out with cotton plants. We identified a total of 23 GhMTP proteins ranged from 371 to 496 amino acids, which were predicted to be located in the cell membrane and vacuole, while most of which have 4 to 6 transmembrane domains. The 23 GhMTPs were divided into 3 clusters by phylogenetic analysis, Mn-CDF, Zn/Fe-CDF, and Zn-CDF clusters. Meanwhile, the promoter region of <i>GhMTPs</i> contains 4282 binding sites associated with 12 transcription factor families. The expression pattern of <i>GhMTPs</i> was relatively stable in anther, bract, filament, leaf, petal, pistil, root, sepal, stem, and torus, indicating that GhMTP genes were functionally conserved in cotton evolution. In addition, we found that <i>GhMTP2</i>, <i>GhMTP3</i>, <i>GhMTP5</i>, <i>GhMTP8</i>, <i>GhMTP15</i>, <i>GhMTP17</i>, <i>GhMTP19</i>, and <i>GhMTP22</i> in the cotton leaves could be induced by specific metal ions. These results provide important information for understanding the heavy metal tolerance mechanism and genetic improvement of cotton, and will be helpful for the phytoremediation of heavy metal contaminated soils in the future.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genome-wide Identification of the MTP Gene Family in Cotton and Expression Analysis Under Multiple Metal Stresses

  • 期刊论文

  • 发表日期：

    2025年3月5日

查看全文
  • Yong Yang,
  • Wenjie Lai,
  • Ping Li,
  • Shihan Zhou,
  • Yutong Wu,
  • Yuanhao Ding,
  • Haiyan Hu

摘要

Metal tolerance proteins (MTPs) play central roles in metal homeostasis in plant cells and tissues. Cotton (Gossypium hirsutum) has strong tolerance to metal stresses and can be used as a phytoremediation crop for heavy metal-contaminated areas. However, the information and biological functions of the MTP gene family in cotton and their roles in metal stresses remain unclear. In this study, a comprehensive analysis of MTP genes was carried out with cotton plants. We identified a total of 23 GhMTP proteins ranged from 371 to 496 amino acids, which were predicted to be located in the cell membrane and vacuole, while most of which have 4 to 6 transmembrane domains. The 23 GhMTPs were divided into 3 clusters by phylogenetic analysis, Mn-CDF, Zn/Fe-CDF, and Zn-CDF clusters. Meanwhile, the promoter region of GhMTPs contains 4282 binding sites associated with 12 transcription factor families. The expression pattern of GhMTPs was relatively stable in anther, bract, filament, leaf, petal, pistil, root, sepal, stem, and torus, indicating that GhMTP genes were functionally conserved in cotton evolution. In addition, we found that GhMTP2, GhMTP3, GhMTP5, GhMTP8, GhMTP15, GhMTP17, GhMTP19, and GhMTP22 in the cotton leaves could be induced by specific metal ions. These results provide important information for understanding the heavy metal tolerance mechanism and genetic improvement of cotton, and will be helpful for the phytoremediation of heavy metal contaminated soils in the future.