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<p>Tall fescue (<i>Festuca arundinacea</i> Schreb.) is a crucial grass species known for its adaptability and durability, integral to agriculture and environmental sustainability. Understanding its genetic diversity is vital for breeding and enhancing ecological adaptability. This study aims to evaluate the genetic diversity of 35 Tunisian accessions of tall fescue, collected in 32 sites and grouped in four geographical regions, compared to the introduced Bullseye French variety, using sequence-related amplified polymorphism (SRAP) markers. A total of 316 high-intensity bands, ranging from 124 to 2392&#xa0;bp, were produced using 14 SRAP primer pair combinations, averaging 22.57 bands per combination. Polymorphic information content (PIC) values ranged from 0.370 to 0.398, and the discrimination power (DP) averaged 0.685, indicating significant genetic diversity. Both UPGMA and STRUCTURE analyses identified two distinct genetic clusters, separating Tunisian accessions from the French variety. Moreover, the genetic differentiation was related mainly to geographical membership with an overall PhiPT (analog of fixation index F<sub>ST</sub>) value of 0.278. AMOVA partition revealed that the most SRAP variation (72.17%) occurred within regions rather than among regions. These results underline the efficacy of SRAP markers in supplying sufficient information in the diversity analysis of tall fescue. This study establishes a foundation for future genetic research and breeding programs to enhance the adaptability and management of tall fescue genetic resources.</p>

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Genetic Diversity Assessment in Tunisian Tall Fescue (Festuca arundinacea Schreb) Using SRAP Markers: Implications for Breeding and Germplasm Conservation

  • 期刊论文

  • 发表日期：

    2025年2月17日

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  • Hala Chadded,
  • Manna Crespan,
  • Karim Guenni,
  • Salma Sai-Kachout,
  • Nidhal Chtourou-Ghorbel

摘要

Tall fescue (Festuca arundinacea Schreb.) is a crucial grass species known for its adaptability and durability, integral to agriculture and environmental sustainability. Understanding its genetic diversity is vital for breeding and enhancing ecological adaptability. This study aims to evaluate the genetic diversity of 35 Tunisian accessions of tall fescue, collected in 32 sites and grouped in four geographical regions, compared to the introduced Bullseye French variety, using sequence-related amplified polymorphism (SRAP) markers. A total of 316 high-intensity bands, ranging from 124 to 2392 bp, were produced using 14 SRAP primer pair combinations, averaging 22.57 bands per combination. Polymorphic information content (PIC) values ranged from 0.370 to 0.398, and the discrimination power (DP) averaged 0.685, indicating significant genetic diversity. Both UPGMA and STRUCTURE analyses identified two distinct genetic clusters, separating Tunisian accessions from the French variety. Moreover, the genetic differentiation was related mainly to geographical membership with an overall PhiPT (analog of fixation index FST) value of 0.278. AMOVA partition revealed that the most SRAP variation (72.17%) occurred within regions rather than among regions. These results underline the efficacy of SRAP markers in supplying sufficient information in the diversity analysis of tall fescue. This study establishes a foundation for future genetic research and breeding programs to enhance the adaptability and management of tall fescue genetic resources.