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Background and Aims <p>The study conducted to evaluate novel diazotrophs to substitute mineral nitrogen fertilizer while improving growth and yield in sugarcane.</p> Methods <p>Standard microbiological methods employed for isolation, characterization, identification and inoculum preparation of diazotrophic bacteria during <i>in-vitro</i> studies. Field experiment conducted over two cropping seasons using randomized block design with seven treatments, comprising inoculation with <i>G. sacchari</i> strain NB2 and <i>G. diazotrophicus</i> strain NB73, combined with 0, 50&#xa0;and 75% of the recommended nitrogen dose (150 N kg ha⁻<sup>1</sup>), along with an un-inoculated control receiving 100% recommended nitrogen fertilizer.</p> Results <p>Among the endophytic diazotrophs, <i>G. sacchari</i> strain NB2 and <i>G. diazotrophicus</i> strain NB73 exhibited high indole-3-acetic acid production (29.3 and 29.2&#xa0;µg&#xa0;ml⁻<sup>1</sup>), phosphate solubilisation (82.9 and 89.6&#xa0;µg&#xa0;ml⁻<sup>1</sup>) and strong nitrogenase activity (≥ 200 n moles C<sub>2</sub>H<sub>4</sub>/hr/mg cell protein), respectively. In field experiments, treatment comprising <i>G. sacchari</i> strain NB2 + 75% N recorded the highest nitrogen uptake (261.65&#xa0;kg&#xa0;ha⁻<sup>1</sup>) and total dry matter at harvest (52.08 t ha⁻<sup>1</sup>), significantly surpassing the un-inoculated control receiving 100% N (210.17&#xa0;kg&#xa0;ha⁻<sup>1</sup> N uptake and 28.57 t ha⁻<sup>1</sup> dry matter accumulation). Treatments receiving <i>G. sacchari</i> strain NB2 in combination with 50 to 75% nitrogen were consistently comparable or superior to the conventional fertilizer regime across both cropping seasons, with most treatment effects being statistically significant.</p> Conclusion <p>The integration of novel endophytic diazotrophs, markedly improved nitrogen acquisition, physiological and yield performance, enabling a reduction of at least 25% in mineral nitrogen fertilizer without yield penalty.</p>

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Integration of novel endophytic diazotrophs with reduced nitrogen fertilization improves nitrogen uptake and productivity in sugarcane in subtropical India

  • 期刊论文

  • 发表日期：

    2026年7月9日

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  • Lalan Sharma,
  • Vijai P. Jaiswal,
  • Sudhir Kr. Shukla,
  • Laxmi Rawat,
  • Asha Gaur

摘要

Background and Aims

The study conducted to evaluate novel diazotrophs to substitute mineral nitrogen fertilizer while improving growth and yield in sugarcane.

Methods

Standard microbiological methods employed for isolation, characterization, identification and inoculum preparation of diazotrophic bacteria during in-vitro studies. Field experiment conducted over two cropping seasons using randomized block design with seven treatments, comprising inoculation with G. sacchari strain NB2 and G. diazotrophicus strain NB73, combined with 0, 50 and 75% of the recommended nitrogen dose (150 N kg ha⁻1), along with an un-inoculated control receiving 100% recommended nitrogen fertilizer.

Results

Among the endophytic diazotrophs, G. sacchari strain NB2 and G. diazotrophicus strain NB73 exhibited high indole-3-acetic acid production (29.3 and 29.2 µg ml⁻1), phosphate solubilisation (82.9 and 89.6 µg ml⁻1) and strong nitrogenase activity (≥ 200 n moles C2H4/hr/mg cell protein), respectively. In field experiments, treatment comprising G. sacchari strain NB2 + 75% N recorded the highest nitrogen uptake (261.65 kg ha⁻1) and total dry matter at harvest (52.08 t ha⁻1), significantly surpassing the un-inoculated control receiving 100% N (210.17 kg ha⁻1 N uptake and 28.57 t ha⁻1 dry matter accumulation). Treatments receiving G. sacchari strain NB2 in combination with 50 to 75% nitrogen were consistently comparable or superior to the conventional fertilizer regime across both cropping seasons, with most treatment effects being statistically significant.

Conclusion

The integration of novel endophytic diazotrophs, markedly improved nitrogen acquisition, physiological and yield performance, enabling a reduction of at least 25% in mineral nitrogen fertilizer without yield penalty.