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Background and aims <p>In facilitative plant-plant interactions, symbiosis play a crucial&#xa0;role in ecosystem functioning through resource sharing. The common mycorrhizal networks (CMNs) are known to play role in nutrient acquisition. Based on our previous observations of CMN integrity fostering tomato biomass and nutrients transfer in tomato-potato onion intercropping system. This experiment investigates how disrupting CMNs modulates transcript abundance of nutrient and hormone transporter genes and phytohormones in tomato within a tomato-potato onion intercropping system.</p> Methods <p>We used Root Exclusion Chambers (RECs) to disrupt the indigenous CMNs and subsequently allowed these symbiotic networks to re-establish. Gene expression related to nutrients and hormones in response to CMN integrity in tomato was analyzed via RT-qPCR, and hormone levels were measured using HPLC.</p> Results <p>Intercropping with potato onion increased IAA and GA3 in tomato roots and showed higher abundance of nutrient transporter genes related to phosphorus (<i>LePT3</i><i>, </i><i>4, and 5)</i>, potassium (Le<i>HAK5</i>), sulphur (<i>Sultr1;1</i> and <i>1;2</i>), iron (<i>IRT1</i> and Le<i>NAS</i>) and zinc (<i>ZIPL</i> and <i>ZIP3L</i>) when CMNs were intact. Disruption of CMNs reduced phytohormone levels and downregulated nutrient transporter genes in tomato, with partial restoration upon re-establishment of CMNs.</p> Conclusion <p>CMN integrity is associated with modulated expression of nutrient transporter genes and phytohormones levels in tomato grown with potato onion, highlighting the potential role of CMNs in influencing nutrient acquisition and hormonal balance in intercropping system, providing a foundation for further mechanistic studies.</p>

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Common mycorrhizal networks regulate nutrient and hormone-related gene expression in tomato under a tomato-potato onion intercropping system

  • 期刊论文

  • 发表日期：

    2026年5月18日

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  • Rahmatullah Khan,
  • Yajie Shang,
  • Chenglin Wang,
  • Fei Sun,
  • Mingzhu Li,
  • Yifan Li,
  • Fengzhi Wu,
  • Danmei Gao

摘要

Background and aims

In facilitative plant-plant interactions, symbiosis play a crucial role in ecosystem functioning through resource sharing. The common mycorrhizal networks (CMNs) are known to play role in nutrient acquisition. Based on our previous observations of CMN integrity fostering tomato biomass and nutrients transfer in tomato-potato onion intercropping system. This experiment investigates how disrupting CMNs modulates transcript abundance of nutrient and hormone transporter genes and phytohormones in tomato within a tomato-potato onion intercropping system.

Methods

We used Root Exclusion Chambers (RECs) to disrupt the indigenous CMNs and subsequently allowed these symbiotic networks to re-establish. Gene expression related to nutrients and hormones in response to CMN integrity in tomato was analyzed via RT-qPCR, and hormone levels were measured using HPLC.

Results

Intercropping with potato onion increased IAA and GA3 in tomato roots and showed higher abundance of nutrient transporter genes related to phosphorus (LePT3, 4, and 5), potassium (LeHAK5), sulphur (Sultr1;1 and 1;2), iron (IRT1 and LeNAS) and zinc (ZIPL and ZIP3L) when CMNs were intact. Disruption of CMNs reduced phytohormone levels and downregulated nutrient transporter genes in tomato, with partial restoration upon re-establishment of CMNs.

Conclusion

CMN integrity is associated with modulated expression of nutrient transporter genes and phytohormones levels in tomato grown with potato onion, highlighting the potential role of CMNs in influencing nutrient acquisition and hormonal balance in intercropping system, providing a foundation for further mechanistic studies.