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Aims <p>The rhizosphere microbiota is critical for crop productivity, but the mechanisms by which host genotype shapes microbial communities to influence yield, especially in legumes, are not well understood. This study aimed to determine whether high-yield pea genotypes are associated with specific beneficial bacteria and whether these microbes can transfer their growth-promoting effects to a low-yield genotype.</p> Methods <p>We investigated four pea (<i>Pisum sativum L.</i>) varieties with contrasting grain-yield phenotypes under field conditions. Rhizosphere microbiomes were characterized using amplicon sequencing and differential abundance analysis, and representative bacterial strains were isolated and functionally characterized in vitro. A greenhouse experiment was then conducted to assess the effects of single and co-inoculation of these strains on the growth and yield of a low-yield pea genotype.</p> Results <p>Two representative strains, <i>Agrobacterium salinitolerans</i> S1 and <i>Neobacillus drentensis</i> S2, were isolated from taxa enriched in high-yield genotypes and both exhibited multiple plant growth-promoting traits in vitro. Reintroduction of these strains into the rhizosphere of a low-yield genotype improved plant growth and grain yield under greenhouse conditions, with co-inoculation showing stronger effects than single-strain treatments.</p> Conclusions <p>Our findings provide direct evidence that high-yield pea genotypes link to beneficial rhizosphere bacteria with transferable growth-promoting effects. This study provides a microbiome-guided framework for identifying candidate beneficial bacteria linked to host genotype and offers a basis for developing genotype-informed microbial strategies to improve pea productivity.</p>

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Host genotype-enriched rhizosphere microbes enhance pea (Pisum sativum L.) yield

  • 期刊论文

  • 发表日期：

    2026年4月25日

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  • Qinwen Yang,
  • Qiong Liu,
  • Ke Dong,
  • Kaile Zhang,
  • Chuanfa Wu,
  • Ruohan Yin,
  • Linying Xu,
  • Zhijuan Feng,
  • Tao Yang,
  • Tida Ge

摘要

Aims

The rhizosphere microbiota is critical for crop productivity, but the mechanisms by which host genotype shapes microbial communities to influence yield, especially in legumes, are not well understood. This study aimed to determine whether high-yield pea genotypes are associated with specific beneficial bacteria and whether these microbes can transfer their growth-promoting effects to a low-yield genotype.

Methods

We investigated four pea (Pisum sativum L.) varieties with contrasting grain-yield phenotypes under field conditions. Rhizosphere microbiomes were characterized using amplicon sequencing and differential abundance analysis, and representative bacterial strains were isolated and functionally characterized in vitro. A greenhouse experiment was then conducted to assess the effects of single and co-inoculation of these strains on the growth and yield of a low-yield pea genotype.

Results

Two representative strains, Agrobacterium salinitolerans S1 and Neobacillus drentensis S2, were isolated from taxa enriched in high-yield genotypes and both exhibited multiple plant growth-promoting traits in vitro. Reintroduction of these strains into the rhizosphere of a low-yield genotype improved plant growth and grain yield under greenhouse conditions, with co-inoculation showing stronger effects than single-strain treatments.

Conclusions

Our findings provide direct evidence that high-yield pea genotypes link to beneficial rhizosphere bacteria with transferable growth-promoting effects. This study provides a microbiome-guided framework for identifying candidate beneficial bacteria linked to host genotype and offers a basis for developing genotype-informed microbial strategies to improve pea productivity.