Scholarlink

Background and aims <p>Continuous strawberry cropping in greenhouse systems often results in soil degradation and rhizosphere dysbiosis, which severely constrain yield stability and sustainable production. Here, we aimed to evaluate how biochar–chemical fertilizer co-application affects soil physicochemical properties, enzyme activities, rhizosphere microbial communities, and strawberry performance in soil collected from a greenhouse continuously cropped with strawberry for six years.</p> Methods <p>A pot experiment was conducted using soil from a greenhouse continuously cropped with strawberry for six years. Treatments included CK (no biochar, no chemical fertilizer), biochar only (T1), two biochar plus chemical fertilizer rates (T2 and T3), and fertilizer only (T4). Rhizosphere soils were collected at the vigorous growth stage to determine moisture, organic matter, pH, TN, TP, TK, and enzyme activities, and bacterial and fungal communities were profiled by 16S rRNA gene (338F/806R) and ITS region (ITS1F/ITS2R) amplicon sequencing.</p> Results <p>The T2 treatment (3% biochar + 0.08% fertilizer) significantly improved soil pH, organic matter, moisture content, and the availability of nitrogen and phosphorus, and produced the greatest increases in leaf chlorophyll content, leaf area, stem diameter, and single-fruit weight. High-throughput sequencing showed that both T2 and T4 markedly increased bacterial α-diversity, reshaped rhizosphere community composition, and enriched putatively beneficial taxa such as Vicinamibacteraceae and <i>Mycothermus</i>. Structural equation modeling supported an integrated association among biochar–fertilizer co-application, soil properties, microbial community structure, and strawberry growth.</p> Conclusion <p>This study suggests that appropriately dosed biochar–fertilizer co-application may help alleviate continuous-cropping constraints in strawberry through coordinated changes in soil properties and rhizosphere microbial communities, and may represent a promising strategy for improving soil conditions and crop performance in protected horticulture.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Biochar–fertilizer synergy mitigates replant disorder by modulating soil nutrients and the rhizosphere microbiome in continuous-cropping strawberry

  • 期刊论文

  • 发表日期：

    2026年4月28日

查看全文
  • Chenbo Jia,
  • Jiayuan Bai,
  • Zheng Luo,
  • Li Tian,
  • Zheng Hou

摘要

Background and aims

Continuous strawberry cropping in greenhouse systems often results in soil degradation and rhizosphere dysbiosis, which severely constrain yield stability and sustainable production. Here, we aimed to evaluate how biochar–chemical fertilizer co-application affects soil physicochemical properties, enzyme activities, rhizosphere microbial communities, and strawberry performance in soil collected from a greenhouse continuously cropped with strawberry for six years.

Methods

A pot experiment was conducted using soil from a greenhouse continuously cropped with strawberry for six years. Treatments included CK (no biochar, no chemical fertilizer), biochar only (T1), two biochar plus chemical fertilizer rates (T2 and T3), and fertilizer only (T4). Rhizosphere soils were collected at the vigorous growth stage to determine moisture, organic matter, pH, TN, TP, TK, and enzyme activities, and bacterial and fungal communities were profiled by 16S rRNA gene (338F/806R) and ITS region (ITS1F/ITS2R) amplicon sequencing.

Results

The T2 treatment (3% biochar + 0.08% fertilizer) significantly improved soil pH, organic matter, moisture content, and the availability of nitrogen and phosphorus, and produced the greatest increases in leaf chlorophyll content, leaf area, stem diameter, and single-fruit weight. High-throughput sequencing showed that both T2 and T4 markedly increased bacterial α-diversity, reshaped rhizosphere community composition, and enriched putatively beneficial taxa such as Vicinamibacteraceae and Mycothermus. Structural equation modeling supported an integrated association among biochar–fertilizer co-application, soil properties, microbial community structure, and strawberry growth.

Conclusion

This study suggests that appropriately dosed biochar–fertilizer co-application may help alleviate continuous-cropping constraints in strawberry through coordinated changes in soil properties and rhizosphere microbial communities, and may represent a promising strategy for improving soil conditions and crop performance in protected horticulture.