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Background and aims <p>Rice is a vital staple crop in China. With climate warming, drought has become a significant threat to rice cultivation. Endophytes have garnered considerable attention for their potential to enhance plant stress resistance, yet their underlying mechanisms remain poorly understood. This study aims to investigate the effects of inoculating <i>Colletotrichum</i> EF0801 strain on the drought tolerance of rice and elucidate the underlying molecular mechanisms.</p> Methods <p>This experiment investigated the growth and physiological indicators of rice seedlings inoculated and non-inoculated with <i>Colletotrichum</i> EF0801 strain under polyethylene glycol 6000-simulated drought stress, and conducted metabolomics analysis.</p> Results <p>Results indicated that under polyethylene glycol stress, the EF0801 strain formed an effective symbiotic relationship with rice seedlings, resulting in increased biomass. Following inoculation with <i>Colletotrichum</i> EF0801 strain, electrical conductivity, malondialdehyde, and H₂O₂ contents of rice seedlings decreased, while proline and ascorbate-glutathione cycle-related substances increased. In addition, the EF0801 strain promoted chlorophyll accumulation, enhanced the maximum photochemical efficiency and photochemical quenching coefficient of photosystem II, and optimized gas exchange parameters. Metabolomics revealed that the tentatively identified differentially accumulated metabolites in rice leaves inoculated with <i>Colletotrichum</i> EF0801 strain under polyethylene glycol stress were primarily enriched in starch and sucrose metabolism, glutathione metabolism pathways. These metabolic pathways and metabolites collectively participate in regulating plant osmotic balance, antioxidant defense, and energy supply, and have responded to environmental stress.</p> Conclusions <p>These findings indicate that <i>Colletotrichum</i> EF0801 strain enhances rice tolerance to polyethylene glycol-induced osmotic stress, providing a basis for further evaluation under actual drought conditions.</p>

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Physiological and metabolomic analyses reveal the potential mechanisms of Colletotrichum gloeosporioides in alleviating osmotic stress in rice

  • 期刊论文

  • 发表日期：

    2026年4月23日

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  • Xinnan Wang,
  • Yuping Jiang,
  • Yueying Li,
  • Lianju Ma,
  • Lanlan Wang,
  • Xuemei Li

摘要

Background and aims

Rice is a vital staple crop in China. With climate warming, drought has become a significant threat to rice cultivation. Endophytes have garnered considerable attention for their potential to enhance plant stress resistance, yet their underlying mechanisms remain poorly understood. This study aims to investigate the effects of inoculating Colletotrichum EF0801 strain on the drought tolerance of rice and elucidate the underlying molecular mechanisms.

Methods

This experiment investigated the growth and physiological indicators of rice seedlings inoculated and non-inoculated with Colletotrichum EF0801 strain under polyethylene glycol 6000-simulated drought stress, and conducted metabolomics analysis.

Results

Results indicated that under polyethylene glycol stress, the EF0801 strain formed an effective symbiotic relationship with rice seedlings, resulting in increased biomass. Following inoculation with Colletotrichum EF0801 strain, electrical conductivity, malondialdehyde, and H₂O₂ contents of rice seedlings decreased, while proline and ascorbate-glutathione cycle-related substances increased. In addition, the EF0801 strain promoted chlorophyll accumulation, enhanced the maximum photochemical efficiency and photochemical quenching coefficient of photosystem II, and optimized gas exchange parameters. Metabolomics revealed that the tentatively identified differentially accumulated metabolites in rice leaves inoculated with Colletotrichum EF0801 strain under polyethylene glycol stress were primarily enriched in starch and sucrose metabolism, glutathione metabolism pathways. These metabolic pathways and metabolites collectively participate in regulating plant osmotic balance, antioxidant defense, and energy supply, and have responded to environmental stress.

Conclusions

These findings indicate that Colletotrichum EF0801 strain enhances rice tolerance to polyethylene glycol-induced osmotic stress, providing a basis for further evaluation under actual drought conditions.