Scholarlink

Aims <p>To quantify multigenerational barley responses to combined cold and drought stress, evaluate heritable resilience, and identify loci and candidate genes controlling stress performance and priming-benefit indices.</p> Methods <p>A barley diversity panel was evaluated across three generations under combined cold and drought stress. Traits included agronomic, phenological, canopy, photosynthetic, water-relation, and redox/oxidative parameters. Stress resilience (SR) and priming benefit (PBI%) were calculated, followed by BLINK-based GWAS with population-structure covariates and RT–qPCR validation of candidate genes in contrasting genotypes.</p> Results <p>Combined cold × drought stress generated a reproducible multigenerational response affecting physiological and biochemical traits. Grain yield and harvest index declined by 31–36% and 12–15%, respectively, whereas heading and maturity were delayed by 3–4%, and stay-green duration decreased by 30%. SPAD and NDVI declined by about 20%, Fv/Fm by 10–12%, and canopy temperature increased by 16–18%. Plant water status was altered, with relative water content reduced by 15–18%, stomatal conductance by about 40%, and leaf water potential becoming more negative. Biochemical changes included higher H₂O₂, MDA, electrolyte leakage, and proline, together with lower GSH/GSSG and AsA/DHA ratios. During the 0–36 h time course, tolerant and susceptible genotypes diverged after stress onset. The tolerant genotype showed earlier induction at 12 h and sustained expression at 36 h for six GWAS-prioritized genes associated with signaling, antioxidant detoxification, ubiquitin regulation, transport/energization, and chloroplast proteostasis.</p> Conclusions <p>Combined cold × drought tolerance involves an integrated hydraulic–redox syndrome, with co-mapping and RT–qPCR highlighting Ψ_leaf- and redox-linked regulators as breeding targets.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Transgenerational redox priming and hormone–ROS crosstalk enhance barley tolerance to combined cold and drought stress

  • 期刊论文

  • 发表日期：

    2026年4月22日

查看全文
  • Modhi O. Alotaibi,
  • Khairiah Mubarak Alwutayd,
  • Ahmad M. Alqudah,
  • Samar G. Thabet

摘要

Aims

To quantify multigenerational barley responses to combined cold and drought stress, evaluate heritable resilience, and identify loci and candidate genes controlling stress performance and priming-benefit indices.

Methods

A barley diversity panel was evaluated across three generations under combined cold and drought stress. Traits included agronomic, phenological, canopy, photosynthetic, water-relation, and redox/oxidative parameters. Stress resilience (SR) and priming benefit (PBI%) were calculated, followed by BLINK-based GWAS with population-structure covariates and RT–qPCR validation of candidate genes in contrasting genotypes.

Results

Combined cold × drought stress generated a reproducible multigenerational response affecting physiological and biochemical traits. Grain yield and harvest index declined by 31–36% and 12–15%, respectively, whereas heading and maturity were delayed by 3–4%, and stay-green duration decreased by 30%. SPAD and NDVI declined by about 20%, Fv/Fm by 10–12%, and canopy temperature increased by 16–18%. Plant water status was altered, with relative water content reduced by 15–18%, stomatal conductance by about 40%, and leaf water potential becoming more negative. Biochemical changes included higher H₂O₂, MDA, electrolyte leakage, and proline, together with lower GSH/GSSG and AsA/DHA ratios. During the 0–36 h time course, tolerant and susceptible genotypes diverged after stress onset. The tolerant genotype showed earlier induction at 12 h and sustained expression at 36 h for six GWAS-prioritized genes associated with signaling, antioxidant detoxification, ubiquitin regulation, transport/energization, and chloroplast proteostasis.

Conclusions

Combined cold × drought tolerance involves an integrated hydraulic–redox syndrome, with co-mapping and RT–qPCR highlighting Ψ_leaf- and redox-linked regulators as breeding targets.