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Background <p>Cadmium (Cd), a highly toxic and mobile heavy metal pollutant, is readily absorbed by plants andinduces oxidative stress, severely impairing plant growth and development. Blue honeysuckle (Lonicera caerulea L.),a resilient woody plant, exhibits notable tolerance under abiotic stress; however, its molecular response mechanismsto Cd remain poorly understood.</p> Objective <p>This study aimed to systematically investigate the physiological, transcriptomic, and metabolomic responses of blue honeysuckle roots to Cd stress and to elucidate the underlying molecular mechanisms governing Cd tolerance.</p> Methods <p>An integrated hydroponic experimental approach was employed to characterize blue honeysuckle root responses under Cd stress. Physiological parameters including antioxidant enzyme activities, lipid peroxidation, and osmoregulatory substance contents were measured. Transcriptome sequencing and metabolome profiling were conducted, followed by integrated multi-omics analysis to identify key pathways and regulatory networks.&#xa0;</p> Results <p>Cd treatment significantly altered the activities of antioxidant enzymes (superoxide dismutase, peroxidase, and catalase), malondialdehyde content, and the levels of osmoregulatory substances (soluble sugars, proline, and glutathione), indicating activation of multi-layered defense mechanisms to mitigate oxidative damage. Transcriptome analysis identified significant upregulation of transcription factor families including WRKY, AP2/ERF-ERF, and Tify. Metabolomic analysis revealed eight differentially accumulated metabolites with elevated levels under Cd stress. Integrated analysis demonstrated that plant signal transduction, glutathione metabolism, and phenylpropanoid biosynthesis are central to Cd resistance in blue honeysuckle.</p> Conclusion <p>Blue honeysuckle employs a coordinated defense strategy involving antioxidant systems, transcriptional reprogramming, and metabolic remodeling to cope with Cd stress. Glutathione metabolism and phenylpropanoid biosynthesis emerge as critical pathways for Cd detoxification and tolerance in this species.</p>

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Integration of physiomics, transcriptomics and metabolomics to study the detoxification mechanism of blue honeysuckle under cadmium stress

  • 期刊论文

  • 发表日期：

    2026年3月2日

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  • Weixia Zhang,
  • Xuelin Zhang,
  • Kexin Liu,
  • Xueying Zhao,
  • Ruiqun Ma,
  • Lin Wang,
  • Peng Zhang,
  • Junwei Huo,
  • Dong Qin

摘要

Background

Cadmium (Cd), a highly toxic and mobile heavy metal pollutant, is readily absorbed by plants andinduces oxidative stress, severely impairing plant growth and development. Blue honeysuckle (Lonicera caerulea L.),a resilient woody plant, exhibits notable tolerance under abiotic stress; however, its molecular response mechanismsto Cd remain poorly understood.

Objective

This study aimed to systematically investigate the physiological, transcriptomic, and metabolomic responses of blue honeysuckle roots to Cd stress and to elucidate the underlying molecular mechanisms governing Cd tolerance.

Methods

An integrated hydroponic experimental approach was employed to characterize blue honeysuckle root responses under Cd stress. Physiological parameters including antioxidant enzyme activities, lipid peroxidation, and osmoregulatory substance contents were measured. Transcriptome sequencing and metabolome profiling were conducted, followed by integrated multi-omics analysis to identify key pathways and regulatory networks. 

Results

Cd treatment significantly altered the activities of antioxidant enzymes (superoxide dismutase, peroxidase, and catalase), malondialdehyde content, and the levels of osmoregulatory substances (soluble sugars, proline, and glutathione), indicating activation of multi-layered defense mechanisms to mitigate oxidative damage. Transcriptome analysis identified significant upregulation of transcription factor families including WRKY, AP2/ERF-ERF, and Tify. Metabolomic analysis revealed eight differentially accumulated metabolites with elevated levels under Cd stress. Integrated analysis demonstrated that plant signal transduction, glutathione metabolism, and phenylpropanoid biosynthesis are central to Cd resistance in blue honeysuckle.

Conclusion

Blue honeysuckle employs a coordinated defense strategy involving antioxidant systems, transcriptional reprogramming, and metabolic remodeling to cope with Cd stress. Glutathione metabolism and phenylpropanoid biosynthesis emerge as critical pathways for Cd detoxification and tolerance in this species.