Scholarlink

Aims <p>Continuous cropping of pepper (<i>Capsicum annuum</i> L.) results in yield decline, but the underlying mechanisms through which rhizosphere metabolites and the microbiome interact remain unclear.</p> Methods <p>In this study, we integrated soil physicochemical analysis, microbial community profiling (16S/ITS), and non-targeted metabolomics after 2, 6, and 10&#xa0;years of continuous cropping (CC2, CC6, and CC10, respectively).</p> Results <p>CC6 soils significantly enriched <i>Sphingomonas</i>, a beneficial bacterium that was positively correlated with available phosphorus. It was recruited by root-exuded glucoerucin and glucosylsphingosine to combat pathogens. But compared with CC6, the contents of these two metabolites were reduced in CC10 soil, which diminished the abundance of <i>Sphingomonas,</i> while the pathogens such as <i>Rigidoporus</i> increased significantly. Nutrient levels (nitrate nitrogen, soil organic carbon, available phosphorus/potassium) were elevated in CC10, likely due to pepper death, resulting in an inability to utilize nutrients.</p> Conclusions <p>Integrated analysis revealed that rhizosphere metabolites drove beneficial microorganism recruitment, alleviating continuous cropping obstacles (CCOs) in CC6. However, the exhaustion of key metabolites fostered pathogen dominance in CC10. Our study thus provides key metabolites and microbes as new targets for the microbial regulation of pepper CCOs.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Rhizosphere metabolite–microbiome synergism mitigates 6 years continuous cropping obstacle in pepper plants

  • 期刊论文

  • 发表日期：

    2026年2月24日

查看全文
  • Xinru Hu,
  • Shancong Wang,
  • Yifei Sun,
  • Fangfang Liang,
  • Fanzheng Meng,
  • Yanling Kou,
  • Xiao Chen,
  • Yulou Tang,
  • Reda M. M. Ahmed,
  • Qinghua Yang,
  • Yu Shi,
  • Xinqi Huang,
  • Ruixin Shao,
  • Nasr M. Abdou

摘要

Aims

Continuous cropping of pepper (Capsicum annuum L.) results in yield decline, but the underlying mechanisms through which rhizosphere metabolites and the microbiome interact remain unclear.

Methods

In this study, we integrated soil physicochemical analysis, microbial community profiling (16S/ITS), and non-targeted metabolomics after 2, 6, and 10 years of continuous cropping (CC2, CC6, and CC10, respectively).

Results

CC6 soils significantly enriched Sphingomonas, a beneficial bacterium that was positively correlated with available phosphorus. It was recruited by root-exuded glucoerucin and glucosylsphingosine to combat pathogens. But compared with CC6, the contents of these two metabolites were reduced in CC10 soil, which diminished the abundance of Sphingomonas, while the pathogens such as Rigidoporus increased significantly. Nutrient levels (nitrate nitrogen, soil organic carbon, available phosphorus/potassium) were elevated in CC10, likely due to pepper death, resulting in an inability to utilize nutrients.

Conclusions

Integrated analysis revealed that rhizosphere metabolites drove beneficial microorganism recruitment, alleviating continuous cropping obstacles (CCOs) in CC6. However, the exhaustion of key metabolites fostered pathogen dominance in CC10. Our study thus provides key metabolites and microbes as new targets for the microbial regulation of pepper CCOs.

Graphical abstract