Scholarlink

Background and Aims <p>Intercropping of desulfurized gypsum with legume green manure is a sustainable strategy to improve soil quality and microbial community in saline-alkali land, but its comprehensive effects on soil physical and chemical properties, enzyme activity and microbial interaction still need to be explored.</p> Methods <p>In a severely saline-alkali soil (Pingluo County, Ningxia, China), four treatments were compared: (i) unamended sorghum monoculture (CK); (ii) desulfurized gypsum amendment (T1); (iii) gypsum + sorghum:&#xa0;Sesbania cannabina 1:1 intercropping (T2); (iv) gypsum + sorghum: Sesbania 3:2 intercropping (T3). Soil physicochemical properties and enzyme activities were measured, and soil microbial communities and functions were characterized by metagenomic sequencing.</p> Results <p>1) T1–T3 reduced total salt content by 55.9–64.0% and soil pH by 1.49–3.40 units compared with CK. Ammonium-N decreased, while total N increased; nitrate–N increased significantly under T2 and T3. Desulfurized gypsum significantly increased the relative abundance of <i>Proteobacteria</i> while decreasing <i>Gemmatimonadota</i>, and intercropping further altered the abundances of <i>Actinobacteria</i>, <i>Chloroflexota</i> and <i>Bacteroidetes</i>. Sulfur metabolism (ko00920) contributed positively to nutrient transformation, whereas glutathione metabolism (ko00480) was associated with improved C/N cycling efficiency through mitigation of oxidative stress. T3 achieved the highest integrated score, mainly driven by higher organic matter, total nitrogen and enzyme activities.</p> Conclusion <p>Together, these results demonstrate that desulfurized gypsum alleviates saline-alkali stress and, when combined with N-fixing <i>Sesbania</i> intercropping, restructures the rhizosphere microbial community and its metabolic pathways to enhance soil nutrient cycling and enzyme activities. Among the four treatments, the T3 (3:2 intercropping) model proved most effective, presenting a viable strategy for ecological restoration of arid saline-alkali regions in Northwest China.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Synergistic improvement of saline-alkali soil quality by desulfurized gypsum and Sesbania cannabina intercropping: Microbial mechanisms and field efficacy

  • 期刊论文

  • 发表日期：

    2026年1月27日

查看全文
  • Linfeng Huang,
  • Zhen Wang,
  • Lirong Chen,
  • Yiqin Feng,
  • Fengju Zhang,
  • Jili Liu,
  • Chengyan Zhang,
  • Lina Wang,
  • Long Chen,
  • Fucheng Guo

摘要

Background and Aims

Intercropping of desulfurized gypsum with legume green manure is a sustainable strategy to improve soil quality and microbial community in saline-alkali land, but its comprehensive effects on soil physical and chemical properties, enzyme activity and microbial interaction still need to be explored.

Methods

In a severely saline-alkali soil (Pingluo County, Ningxia, China), four treatments were compared: (i) unamended sorghum monoculture (CK); (ii) desulfurized gypsum amendment (T1); (iii) gypsum + sorghum: Sesbania cannabina 1:1 intercropping (T2); (iv) gypsum + sorghum: Sesbania 3:2 intercropping (T3). Soil physicochemical properties and enzyme activities were measured, and soil microbial communities and functions were characterized by metagenomic sequencing.

Results

1) T1–T3 reduced total salt content by 55.9–64.0% and soil pH by 1.49–3.40 units compared with CK. Ammonium-N decreased, while total N increased; nitrate–N increased significantly under T2 and T3. Desulfurized gypsum significantly increased the relative abundance of Proteobacteria while decreasing Gemmatimonadota, and intercropping further altered the abundances of Actinobacteria, Chloroflexota and Bacteroidetes. Sulfur metabolism (ko00920) contributed positively to nutrient transformation, whereas glutathione metabolism (ko00480) was associated with improved C/N cycling efficiency through mitigation of oxidative stress. T3 achieved the highest integrated score, mainly driven by higher organic matter, total nitrogen and enzyme activities.

Conclusion

Together, these results demonstrate that desulfurized gypsum alleviates saline-alkali stress and, when combined with N-fixing Sesbania intercropping, restructures the rhizosphere microbial community and its metabolic pathways to enhance soil nutrient cycling and enzyme activities. Among the four treatments, the T3 (3:2 intercropping) model proved most effective, presenting a viable strategy for ecological restoration of arid saline-alkali regions in Northwest China.