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Background and aims <p>Soil microorganisms play a pivotal role in modulating plant nutrient uptake, yet the influence of soil yeasts on nitrate transporter dynamics remains largely unexplored. This study examines the effects of two rhizosphere yeasts—<i>Solicoccozyma terrea</i>&#xa0;(a yeast strain with ACC-deaminase activity) and&#xa0;<i>Metschnikowia sp.</i>&#xa0;(indole-3-acetic acid producer)—on the expression and tissue localization of three nitrate transporters (NRT1.2, NRT2.1, and NRT2.3) in tomato (<i>Solanum lycopersicum</i>) roots.</p> Methods <p>Plant roots were exposed to both, individually or in combination, yeast strains under controlled conditions. Gene expression analysis via RT-qPCR was performed every two days after exposure, and after 20&#xa0;days, stem samples were collected to determine nitrate content in the xylem sap. In a second experiment, plants were transformed to express the nitrate transporters of interest fused to NeonGreen. After 20&#xa0;days of exposure to the yeast strains, root samples were collected and analyzed by confocal microscopy.</p> Results <p>The expression of the high-affinity nitrate transporters NRT2.1 and NRT2.3 was upregulated by <i>S. terrea</i>, while&#xa0;<i>Metschnikowia sp.</i>&#xa0;induced transient expression of the dual-affinity LeNRT1.2. Confocal imaging showed yeast-specific shifts in transporter localization, with&#xa0;<i>S. terrea</i>&#xa0;enhancing vascular bundle accumulation and&#xa0;<i>Metschnikowia sp.</i>&#xa0;promoting epidermal presence of NRT2.3.</p> Conclusions <p>These findings suggest that soil yeasts can modulate nitrate acquisition pathways through distinct biochemical signaling, offering new perspectives on microbiome-driven strategies to improve nitrogen use efficiency in horticultural systems.</p>

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Root nitrate transporters expression and localization in tomato respond differently to soil yeasts with ACC deaminase activity or indole-3-acetic acid synthesis

  • 期刊论文

  • 发表日期：

    2026年1月29日

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  • Francisco Albornoz,
  • Marlene Gebauer,
  • Daniela Catrileo,
  • Mariajosé Carvajal,
  • Liliana Godoy

摘要

Background and aims

Soil microorganisms play a pivotal role in modulating plant nutrient uptake, yet the influence of soil yeasts on nitrate transporter dynamics remains largely unexplored. This study examines the effects of two rhizosphere yeasts—Solicoccozyma terrea (a yeast strain with ACC-deaminase activity) and Metschnikowia sp. (indole-3-acetic acid producer)—on the expression and tissue localization of three nitrate transporters (NRT1.2, NRT2.1, and NRT2.3) in tomato (Solanum lycopersicum) roots.

Methods

Plant roots were exposed to both, individually or in combination, yeast strains under controlled conditions. Gene expression analysis via RT-qPCR was performed every two days after exposure, and after 20 days, stem samples were collected to determine nitrate content in the xylem sap. In a second experiment, plants were transformed to express the nitrate transporters of interest fused to NeonGreen. After 20 days of exposure to the yeast strains, root samples were collected and analyzed by confocal microscopy.

Results

The expression of the high-affinity nitrate transporters NRT2.1 and NRT2.3 was upregulated by S. terrea, while Metschnikowia sp. induced transient expression of the dual-affinity LeNRT1.2. Confocal imaging showed yeast-specific shifts in transporter localization, with S. terrea enhancing vascular bundle accumulation and Metschnikowia sp. promoting epidermal presence of NRT2.3.

Conclusions

These findings suggest that soil yeasts can modulate nitrate acquisition pathways through distinct biochemical signaling, offering new perspectives on microbiome-driven strategies to improve nitrogen use efficiency in horticultural systems.