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Background and aims <p>Banana Fusarium wilt, caused by <i>Fusarium oxysporum</i>, is a highly destructive disease that threatens global banana production Biocontrol agents such as <i>Pseudomonas chlororaphis</i> P1 provide a sustainable strategy for disease management. However, how these agent influence indigenous rhizosphere microbiota under varying pathogen pressures remains elusive. This study evaluated the effects of <i>P. chlororaphis</i> P1 on the banana rhizosphere bacterial community across different <i>F. oxysporum</i> infection levels,</p> Methods <p>High-throughput sequencing and quantitative PCR were employed to analyse rhizosphere soils following inoculation with <i>P. chlororaphis</i> P1.</p> Results <p>In soils heavily infested with <i>F. oxysporum</i>, <i>P. chlororaphis</i> P1 exerted no significant effect on community composition. In contrast, in soils with low pathogen levels, the bacterial abundance decreased markedly, and the structure and composition of the bacterial community underwent significant alteration. Notably, the genera <i>Massilia</i> and <i>Pseudoxanthomonas</i> played dual ecological roles: they were significantly negative correlation with <i>Fusarium</i> spp, but a significantly positive correlation with <i>P. chlororaphis</i> P1 (<i>p</i> &lt; 0.05). A significant negative correlation was also observed between the relative abundance of <i>P. chlororaphis</i> P1 and banana wilt disedse severity.</p> Conclusion <p>This study demonstrated that <i>Pseudomonas chlororaphis</i> P1 possesses strong biocontrol efficacy against <i>Fusarium</i> wilt. However, in soils with low <i>Fusarium</i> abundance, P1 application reduced bacterial richness and diversity. <b>These findings</b> highlight the effectiveness of <i>P. chlororaphis</i> P1 for <i>Fusarium</i> wilt suppression, while underscoring the necessity to balance its disease control benefits against potential ecological impacts on endogenous soil microbial communities.</p>

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Effects of Pseudomonas chlororaphis P1 on banana rhizosphere microbiota and Fusarium wilt suppression

  • 期刊论文

  • 发表日期：

    2026年1月17日

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  • Xiuli Song,
  • Jingwen Zhao,
  • Pinmiao Zhang,
  • Hui Zhao,
  • Huixia Chen,
  • Chengyang Li,
  • Qi Liu,
  • Yanpo Yao

摘要

Background and aims

Banana Fusarium wilt, caused by Fusarium oxysporum, is a highly destructive disease that threatens global banana production Biocontrol agents such as Pseudomonas chlororaphis P1 provide a sustainable strategy for disease management. However, how these agent influence indigenous rhizosphere microbiota under varying pathogen pressures remains elusive. This study evaluated the effects of P. chlororaphis P1 on the banana rhizosphere bacterial community across different F. oxysporum infection levels,

Methods

High-throughput sequencing and quantitative PCR were employed to analyse rhizosphere soils following inoculation with P. chlororaphis P1.

Results

In soils heavily infested with F. oxysporum, P. chlororaphis P1 exerted no significant effect on community composition. In contrast, in soils with low pathogen levels, the bacterial abundance decreased markedly, and the structure and composition of the bacterial community underwent significant alteration. Notably, the genera Massilia and Pseudoxanthomonas played dual ecological roles: they were significantly negative correlation with Fusarium spp, but a significantly positive correlation with P. chlororaphis P1 (p < 0.05). A significant negative correlation was also observed between the relative abundance of P. chlororaphis P1 and banana wilt disedse severity.

Conclusion

This study demonstrated that Pseudomonas chlororaphis P1 possesses strong biocontrol efficacy against Fusarium wilt. However, in soils with low Fusarium abundance, P1 application reduced bacterial richness and diversity. These findings highlight the effectiveness of P. chlororaphis P1 for Fusarium wilt suppression, while underscoring the necessity to balance its disease control benefits against potential ecological impacts on endogenous soil microbial communities.