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Background and aims <p>Damping-off is a worldwide disease caused by <i>Fusarium oxysporum</i> in forest nurseries. The genera <i>Bacillus</i> and <i>Streptomyces</i>, widely used as biological control agents for damping-off, have rarely been reported in combination. This study aimed to screen the compatibly interactive <i>Bacillus</i> sp. and <i>Streptomyces</i> sp. consortium to control damping-off in pine seedlings and to elucidate its biocontrol mechanisms.</p> Methods <p>We selected optimal bacterial consortium through <i>in vitro</i> and pot experiments, and investigated the colonization ability of strains in plant rhizosphere and the effect of bacterial consortium on soil microbiome by quantitative real-time PCR (qPCR) and amplicon sequencing, respectively.</p> Results <p><i>In vitro</i> experiments revealed that <i>Streptomyces salinarius</i> Lnu-12 and <i>Bacillus halotolerans</i> Lnu-20 strongly inhibited the growth of <i>F. oxysporum</i> and exhibited mutual compatibility<i>.</i> Notably, biofilm formation of Lnu-20 was enhanced by Lnu-12, and Lnu-12 spores were dispersed and transported to plant tissues by Lnu-20. The pot experiments indicated that bacterial consortium displayed superior control efficacy against damping off of pine seedlings, and significantly promoted the growth of pine seedlings. Furthermore, the bacterial consortium treatment enriched the abundance of Lnu-12 and Lnu-20 while reducing <i>F. oxysporum</i> in the pine seedling rhizosphere soil, and shaped the microbial community assembly and function and recruited specific taxa.</p> Conclusion <p>The bacterial consortium of Lnu-12 and Lnu-20 could effectively controlled the damping-off disease and promoted the growth of pine seedlings by synergistic interactions and rhizosphere soil microbiome alterations. This knowledge provides new insights into efficient probiotic consortia design including <i>Bacillus</i> and <i>Streptomyces</i> strains.</p>

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A synthetic biocontrol consortium of Streptomyces salinarius and Bacillus halotolerans exhibits superior protection against Fusarium oxysporum infection in Pinus via synergistic interactions

  • 期刊论文

  • 发表日期：

    2025年12月12日

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  • Haixia Wang,
  • Lin Fu,
  • Chunli Li,
  • Xuanyi Meng,
  • Siyang Wang,
  • Xinlu Zhang,
  • Xinyu Zhou,
  • Xiangna Wang,
  • Fangliang Zheng,
  • Chunyu Zhu

摘要

Background and aims

Damping-off is a worldwide disease caused by Fusarium oxysporum in forest nurseries. The genera Bacillus and Streptomyces, widely used as biological control agents for damping-off, have rarely been reported in combination. This study aimed to screen the compatibly interactive Bacillus sp. and Streptomyces sp. consortium to control damping-off in pine seedlings and to elucidate its biocontrol mechanisms.

Methods

We selected optimal bacterial consortium through in vitro and pot experiments, and investigated the colonization ability of strains in plant rhizosphere and the effect of bacterial consortium on soil microbiome by quantitative real-time PCR (qPCR) and amplicon sequencing, respectively.

Results

In vitro experiments revealed that Streptomyces salinarius Lnu-12 and Bacillus halotolerans Lnu-20 strongly inhibited the growth of F. oxysporum and exhibited mutual compatibility. Notably, biofilm formation of Lnu-20 was enhanced by Lnu-12, and Lnu-12 spores were dispersed and transported to plant tissues by Lnu-20. The pot experiments indicated that bacterial consortium displayed superior control efficacy against damping off of pine seedlings, and significantly promoted the growth of pine seedlings. Furthermore, the bacterial consortium treatment enriched the abundance of Lnu-12 and Lnu-20 while reducing F. oxysporum in the pine seedling rhizosphere soil, and shaped the microbial community assembly and function and recruited specific taxa.

Conclusion

The bacterial consortium of Lnu-12 and Lnu-20 could effectively controlled the damping-off disease and promoted the growth of pine seedlings by synergistic interactions and rhizosphere soil microbiome alterations. This knowledge provides new insights into efficient probiotic consortia design including Bacillus and Streptomyces strains.