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Background and aims <p>Compost-mediated suppression of <i>Ralstonia solanacearum</i>-induced bacterial wilt varies widely among studies and applications, potentially linked to stochastic resurgence of mesophilic bacteria post-thermophilic phase.</p> Methods <p>In this study, compost was amended with soil from seven distinct environments applied in post-thermophilic phase. The resulting compost was iteratively reused as an inoculant across three composting cycles, with bacterial wilt suppression evaluated in the final cycle. Bacterial community dynamics were tracked by 16S <i>rRNA</i> sequencing across source soils, compost from three batches sampled at four time points, and the rhizosphere of tomato plants amended with four composts.</p> Results <p>Microbiomes from the disease-suppressive soil and Wang Mountain increased the suppression of bacterial wilt (<i>R. solanacearum</i>) in compost by 63.1% and 24.6%, respectively. Nevertheless, the compost microbiome exhibited significant batch variability and was influenced more by maturation than by inoculation with soil or compost microbiomes. Despite distinct bacterial communities in soil, compost, and rhizosphere samples, eight ASVs—five Bacilli and three with Actinobacteria—were consistently detected in the disease-suppressive soil, related compost, and the rhizosphere samples. However, the relative abundance of these ASVs was lower in the rhizosphere. Compost inoculated with a microbial consortium of in vitro antagonists showed a 17% improvment in bacterial wilt suppression, despite the antagonists accounting for less than 0.2% of the rhizosphere community.</p> Conclusions <p>In conclusion, the disease-suppressive soil microbiome did not shift dominant compost bacterial communities but may have influenced rare microbial populations, potentially enhancing the compost's efficacy against bacterial wilt.</p>

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Engineering bacterial wilt-suppressive compost through soil microbiome transplantation

  • 期刊论文

  • 发表日期：

    2025年11月28日

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  • Jing Mao,
  • Yu Shi,
  • Zixiu Liu,
  • Fei Wang,
  • Yanran Zhang,
  • Yudan Zhang,
  • Xiaoyan Ding,
  • Ning Wang,
  • Ji Li,
  • Yuquan Wei,
  • Guo-chun Ding

摘要

Background and aims

Compost-mediated suppression of Ralstonia solanacearum-induced bacterial wilt varies widely among studies and applications, potentially linked to stochastic resurgence of mesophilic bacteria post-thermophilic phase.

Methods

In this study, compost was amended with soil from seven distinct environments applied in post-thermophilic phase. The resulting compost was iteratively reused as an inoculant across three composting cycles, with bacterial wilt suppression evaluated in the final cycle. Bacterial community dynamics were tracked by 16S rRNA sequencing across source soils, compost from three batches sampled at four time points, and the rhizosphere of tomato plants amended with four composts.

Results

Microbiomes from the disease-suppressive soil and Wang Mountain increased the suppression of bacterial wilt (R. solanacearum) in compost by 63.1% and 24.6%, respectively. Nevertheless, the compost microbiome exhibited significant batch variability and was influenced more by maturation than by inoculation with soil or compost microbiomes. Despite distinct bacterial communities in soil, compost, and rhizosphere samples, eight ASVs—five Bacilli and three with Actinobacteria—were consistently detected in the disease-suppressive soil, related compost, and the rhizosphere samples. However, the relative abundance of these ASVs was lower in the rhizosphere. Compost inoculated with a microbial consortium of in vitro antagonists showed a 17% improvment in bacterial wilt suppression, despite the antagonists accounting for less than 0.2% of the rhizosphere community.

Conclusions

In conclusion, the disease-suppressive soil microbiome did not shift dominant compost bacterial communities but may have influenced rare microbial populations, potentially enhancing the compost's efficacy against bacterial wilt.