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Background and aims <p>Plant growth-promoting rhizobacteria (PGPR) play a vital role in sustainable agriculture, as they can stimulate plant growth through various mechanisms.</p> Methods <p>Genomic prediction was integrated with functional analysis to explore the potential functional genes of <i>Microbacterium</i> C5 in plant growth promotion and abiotic stress alleviation. The I-plate and pot experiments were conducted to validate the capabilities of the volatile organic compounds (VOCs) produced by <i>Microbacterium</i> C5 (C5-VOCs) to stimulate plant growth. To further elucidate the components of C5-VOCs, the culture extract of <i>Microbacterium</i> C5 was analyzed using Headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME/GC–MS).</p> Results <p>Whole-genome sequencing showed that <i>Microbacterium</i> C5 was a new PGPR, with a genome size of 3,962,105 and an average GC content of 70.4%. <i>Microbacterium</i> C5 had multiple key genes related to IAA production, nitrogen generation, phosphate solubilization, potassium dissolution, siderophore synthesis, salt stress resistance, and heavy metal stress resistance. Furthermore, volatiles of <i>Microbacterium</i> C5 induced significant increases in plant biomass of <i>Arabidopsis thaliana</i> and cotton. Further experiment showed that the exposure of cotton roots to C5-VOCs led to an increase of 97.65% in plant chlorophyll content. HS-SPME/GC–MS analysis revealed that dimethyl disulfide was a major component of C5-VOCs, and the growth responses of cotton to dimethyl disulfide were concentration dependent.</p> Conclusion <p><i>Microbacterium</i> C5 had multifarious plant growth-promoting properties. The research results will be helpful for further studies on the ecological functions and the potential biotechnological applications of PGPR.</p>

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Complete genomic sequence analysis of Microbacterium C5 and the growth-regulating effects of its volatile organic compounds (VOCs) on plants

  • 期刊论文

  • 发表日期：

    2025年11月27日

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  • Hong Chen,
  • YaChao Chi,
  • Jiali Zhang,
  • Yan Sun,
  • Yu Lu,
  • Fenghua Zhang

摘要

Background and aims

Plant growth-promoting rhizobacteria (PGPR) play a vital role in sustainable agriculture, as they can stimulate plant growth through various mechanisms.

Methods

Genomic prediction was integrated with functional analysis to explore the potential functional genes of Microbacterium C5 in plant growth promotion and abiotic stress alleviation. The I-plate and pot experiments were conducted to validate the capabilities of the volatile organic compounds (VOCs) produced by Microbacterium C5 (C5-VOCs) to stimulate plant growth. To further elucidate the components of C5-VOCs, the culture extract of Microbacterium C5 was analyzed using Headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME/GC–MS).

Results

Whole-genome sequencing showed that Microbacterium C5 was a new PGPR, with a genome size of 3,962,105 and an average GC content of 70.4%. Microbacterium C5 had multiple key genes related to IAA production, nitrogen generation, phosphate solubilization, potassium dissolution, siderophore synthesis, salt stress resistance, and heavy metal stress resistance. Furthermore, volatiles of Microbacterium C5 induced significant increases in plant biomass of Arabidopsis thaliana and cotton. Further experiment showed that the exposure of cotton roots to C5-VOCs led to an increase of 97.65% in plant chlorophyll content. HS-SPME/GC–MS analysis revealed that dimethyl disulfide was a major component of C5-VOCs, and the growth responses of cotton to dimethyl disulfide were concentration dependent.

Conclusion

Microbacterium C5 had multifarious plant growth-promoting properties. The research results will be helpful for further studies on the ecological functions and the potential biotechnological applications of PGPR.