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Background and aims <p>Hydrogen sulfide (H₂S) is a gasotransmitter that plays an important physiological role in both plants and animals. For example, specific H₂S-generating enzymes in plant cells have been cloned using analogical methods based on two endogenous H₂S-generating enzymes found in animals, cystathionine γ-lyase (CSE) and cystathionine β-synthase (CBS). However, to date no such studies of homologous genes of the key H₂S-generating enzyme mercaptopyruvate sulfurtransferase (MST), also present in animals, have yet been performed in plants.</p> Methods <p>We cloned two transcripts of <i>AtMST1</i>, designated <i>AtMST1.1</i> and <i>AtMST1.3</i>. Subsequently, <i>AtMST1</i> was expressed in prokaryotes in vitro, and both overexpressing and RNA interference (RNAi) plant lines were generated in <i>Arabidopsis thaliana</i> Col-0.</p> Results <p>Both in vivo and in vitro experimental analyses confirmed the catalytic capacity of AtMST1 to produce H₂S. Moreover, under drought stress, overexpressing of <i>AtMST1</i> significantly enhanced drought tolerance relative to wild-type (WT) plants. Importantly, this was correlated with increased H₂S content and production rate. In contrast, RNAi-<i>AtMST1</i> plants exhibited pronounced leaf wilting, reduced drought tolerance, and a significant decline in H₂S content and production rate. Taken together, these findings highlight the pivotal role of AtMST1 in modulating H₂S levels under drought stress.</p> Conclusions <p>AtMST1 is a novel endogenous H₂S-generating enzyme in plants that enhances drought tolerate.</p>

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AtMST1: a novel hydrogen sulfide-producing enzyme that enhances drought tolerance in Arabidopsis

  • 期刊论文

  • 发表日期：

    2025年11月17日

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  • Xuefeng Hao,
  • Haiyan Cao,
  • Mengjie Xie,
  • Yingjie Yuan,
  • Liping Zhang,
  • Zhuping Jin,
  • Yanxi Pei

摘要

Background and aims

Hydrogen sulfide (H₂S) is a gasotransmitter that plays an important physiological role in both plants and animals. For example, specific H₂S-generating enzymes in plant cells have been cloned using analogical methods based on two endogenous H₂S-generating enzymes found in animals, cystathionine γ-lyase (CSE) and cystathionine β-synthase (CBS). However, to date no such studies of homologous genes of the key H₂S-generating enzyme mercaptopyruvate sulfurtransferase (MST), also present in animals, have yet been performed in plants.

Methods

We cloned two transcripts of AtMST1, designated AtMST1.1 and AtMST1.3. Subsequently, AtMST1 was expressed in prokaryotes in vitro, and both overexpressing and RNA interference (RNAi) plant lines were generated in Arabidopsis thaliana Col-0.

Results

Both in vivo and in vitro experimental analyses confirmed the catalytic capacity of AtMST1 to produce H₂S. Moreover, under drought stress, overexpressing of AtMST1 significantly enhanced drought tolerance relative to wild-type (WT) plants. Importantly, this was correlated with increased H₂S content and production rate. In contrast, RNAi-AtMST1 plants exhibited pronounced leaf wilting, reduced drought tolerance, and a significant decline in H₂S content and production rate. Taken together, these findings highlight the pivotal role of AtMST1 in modulating H₂S levels under drought stress.

Conclusions

AtMST1 is a novel endogenous H₂S-generating enzyme in plants that enhances drought tolerate.