Scholarlink

Background <p>The contribution of ammonia–oxidizing archaea (AOA), bacteria (AOB), and complete ammonia oxidizers (comammox) to nitrous oxide (N<sub>2</sub>O) production following afforestation remains poorly understood, hindering our ability to assess the sustainability of rehabilitated ecosystems.</p> Methods <p>We investigated the factors influencing aerobic N<sub>2</sub>O production in cropland and plantation forest soils in southwest China using molecular techniques and inhibitor experiments, and analysis of soil physicochemical properties across 13 paired sites.</p> Results <p>Our findings reveal that afforestation significantly increased N<sub>2</sub>O production derived from AOA, AOB, and comammox. This increase appeared to be attributed to enhanced N mineralization and ammonia availability, likely driven by elevated exchangeable calcium concentration, alleviated microbial phosphorus limitation, and aggravated nitrogen limitation. Furthermore, we observed that N<sub>2</sub>O production was predominantly mediated by AOB at both land–use types, accounting for 42–47%, followed by AOA (34–37%). In contrast, comammox had a minor contribution to N<sub>2</sub>O emissions, accounting for 10–11%, with an N<sub>2</sub>O yield of approximately 0.04% to 0.22%.</p> Conclusions <p>The positive impacts of afforestation on ammonia oxidizers produced N<sub>2</sub>O should be fully considered when assessing its climate change mitigation outcomes and ecosystem sustainability. We highlight that changes in microbial resource limitation, driven by factors such as calcium availability, are key drivers of altered N<sub>2</sub>O dynamics post-afforestation, rather than soil pH or direct ammonia availability alone.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Afforestation-driven microbial nitrogen limitation promotes soil nitrous oxide production by ammonia oxidizers

  • 期刊论文

  • 发表日期：

    2025年8月23日

查看全文
  • Yuliang Pan,
  • Jianyu Dang,
  • Peng Li,
  • Xinyi Yang,
  • Kongcao Xiao,
  • Kelin Wang,
  • Pengpeng Duan,
  • Dejun Li

摘要

Background

The contribution of ammonia–oxidizing archaea (AOA), bacteria (AOB), and complete ammonia oxidizers (comammox) to nitrous oxide (N2O) production following afforestation remains poorly understood, hindering our ability to assess the sustainability of rehabilitated ecosystems.

Methods

We investigated the factors influencing aerobic N2O production in cropland and plantation forest soils in southwest China using molecular techniques and inhibitor experiments, and analysis of soil physicochemical properties across 13 paired sites.

Results

Our findings reveal that afforestation significantly increased N2O production derived from AOA, AOB, and comammox. This increase appeared to be attributed to enhanced N mineralization and ammonia availability, likely driven by elevated exchangeable calcium concentration, alleviated microbial phosphorus limitation, and aggravated nitrogen limitation. Furthermore, we observed that N2O production was predominantly mediated by AOB at both land–use types, accounting for 42–47%, followed by AOA (34–37%). In contrast, comammox had a minor contribution to N2O emissions, accounting for 10–11%, with an N2O yield of approximately 0.04% to 0.22%.

Conclusions

The positive impacts of afforestation on ammonia oxidizers produced N2O should be fully considered when assessing its climate change mitigation outcomes and ecosystem sustainability. We highlight that changes in microbial resource limitation, driven by factors such as calcium availability, are key drivers of altered N2O dynamics post-afforestation, rather than soil pH or direct ammonia availability alone.