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Background and aims <p>Continuous cropping obstacles (CCOs) are frequently encountered during the cultivation of <i>Lagenaria siceraria</i> (<i>L. siceraria</i>) in the same field for many years, which is related to the secondary metabolites secreted by plants, among which vanillin is one of the factors causing CCOs of <i>L. siceraria</i>. This study investigated the effects of different concentrations of exogenous brassinolide (BR) on L. siceraria under CCOs.</p> Methods <p>High-performance liquid chromatography (HPLC) was used to determine the contents of vanillin in rhizosphere soil of non-planted soil, 1-year-old, 2-year-old, and 3-year-old <i>L. siceraria</i> cultivation. This study investigated the effects of BR at concentrations of 0.05, 0.10, 0.20 and 0.40 mg/mL on <i>L. siceraria</i> under CCOs by applying 6.00 mg/mL vanillin to simulate CCOs.</p> Results <p>The contents of vanillin in the rhizosphere soil of non-planted soil, 1-year-old, 2-year-old, and 3-year-old were 0.01, 0.03, 0.06 and 0.10 mg/g. The BR could effectively alleviate the stress imposed by vanillin and enhance the tolerance of <i>L. siceraria</i> to vanillin stress. When the concentration of BR was 0.20 mg/mL, the alleviation effect on vanillin stress was the most significant. Compared with the vanillin stress group, the plant height, the projected area of the root, number of tips, and total root length enhanced by 1.52, 4.21, 4.43, and 6.12 times. When the light intensity was 1200 lx, the transpiration rate and stomatal conductance increased by 68.57% and 48.00%. At the same time, the antioxidant enzyme activities had the best alleviation at 0.20 mg/mL.</p> Conclusion <p>The vanillin significantly inhibited the growth of <i>L. siceraria</i> seedlings at elevated concentrations. Furthermore, its persistent accumulation in the soil via root exudation was identified as a contributing factor to CCOs. It was worth noting that 0.20 mg/mL BR could alleviate the damage caused by CCOs to <i>L. siceraria</i> seedlings.</p>

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Exogenous brassinolide alleviates the autotoxicity induced by vanillin in Lagenaria siceraria

  • 期刊论文

  • 发表日期：

    2025年6月5日

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  • Yiyue Jiang,
  • Xiaodi Zhang,
  • Hongmei Mu,
  • Shan Liu,
  • Saike Qi,
  • Jinlei Wang,
  • Shengxin Wei,
  • Yixue Wang

摘要

Background and aims

Continuous cropping obstacles (CCOs) are frequently encountered during the cultivation of Lagenaria siceraria (L. siceraria) in the same field for many years, which is related to the secondary metabolites secreted by plants, among which vanillin is one of the factors causing CCOs of L. siceraria. This study investigated the effects of different concentrations of exogenous brassinolide (BR) on L. siceraria under CCOs.

Methods

High-performance liquid chromatography (HPLC) was used to determine the contents of vanillin in rhizosphere soil of non-planted soil, 1-year-old, 2-year-old, and 3-year-old L. siceraria cultivation. This study investigated the effects of BR at concentrations of 0.05, 0.10, 0.20 and 0.40 mg/mL on L. siceraria under CCOs by applying 6.00 mg/mL vanillin to simulate CCOs.

Results

The contents of vanillin in the rhizosphere soil of non-planted soil, 1-year-old, 2-year-old, and 3-year-old were 0.01, 0.03, 0.06 and 0.10 mg/g. The BR could effectively alleviate the stress imposed by vanillin and enhance the tolerance of L. siceraria to vanillin stress. When the concentration of BR was 0.20 mg/mL, the alleviation effect on vanillin stress was the most significant. Compared with the vanillin stress group, the plant height, the projected area of the root, number of tips, and total root length enhanced by 1.52, 4.21, 4.43, and 6.12 times. When the light intensity was 1200 lx, the transpiration rate and stomatal conductance increased by 68.57% and 48.00%. At the same time, the antioxidant enzyme activities had the best alleviation at 0.20 mg/mL.

Conclusion

The vanillin significantly inhibited the growth of L. siceraria seedlings at elevated concentrations. Furthermore, its persistent accumulation in the soil via root exudation was identified as a contributing factor to CCOs. It was worth noting that 0.20 mg/mL BR could alleviate the damage caused by CCOs to L. siceraria seedlings.